Impact of lignins isolated from pretreated lignocelluloses on enzymatic cellulose saccharification
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Impact of lignins isolated from pretreated lignocelluloses on enzymatic cellulose saccharification. / Barsberg, Søren Talbro; Selig, Michael Joseph; Felby, Claus.
In: Biotechnology Letters, Vol. 35, No. 2, 2013, p. 189-195.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Impact of lignins isolated from pretreated lignocelluloses on enzymatic cellulose saccharification
AU - Barsberg, Søren Talbro
AU - Selig, Michael Joseph
AU - Felby, Claus
PY - 2013
Y1 - 2013
N2 - Lignins were enzymatically isolated from corn stover and wheat straw samples and subjected to hydrothermal or wet oxidation pretreatments for enzyme adsorption experimentations. Lignin contents of the isolates ranged from 26 to 71 % (w/w); cellulose ranged from 3 to 22 % (w/w); xylan from 0.7 to 6 % (w/w) and ash was from 5.8 to 30 % (w/w). ATR-IR analyses indicated significant and similar levels of calcium in all lignin isolates. Commercial cellulase adsorption studies showed that the presence of these lignins had no significant impact on the total amount of adsorbed enzyme in cellulose and cellulose-lignin systems. Consequently, the presence of the lignins had minimal effect, if any, on enzymatic cellulose conversion. Furthermore, this result, coupled with significant calcium levels in the isolated lignins, supports previous work suggesting lignin-calcium complexes reduce enzyme-lignin interactions.
AB - Lignins were enzymatically isolated from corn stover and wheat straw samples and subjected to hydrothermal or wet oxidation pretreatments for enzyme adsorption experimentations. Lignin contents of the isolates ranged from 26 to 71 % (w/w); cellulose ranged from 3 to 22 % (w/w); xylan from 0.7 to 6 % (w/w) and ash was from 5.8 to 30 % (w/w). ATR-IR analyses indicated significant and similar levels of calcium in all lignin isolates. Commercial cellulase adsorption studies showed that the presence of these lignins had no significant impact on the total amount of adsorbed enzyme in cellulose and cellulose-lignin systems. Consequently, the presence of the lignins had minimal effect, if any, on enzymatic cellulose conversion. Furthermore, this result, coupled with significant calcium levels in the isolated lignins, supports previous work suggesting lignin-calcium complexes reduce enzyme-lignin interactions.
UR - http://www.scopus.com/inward/record.url?scp=84872801952&partnerID=8YFLogxK
U2 - 10.1007/s10529-012-1061-x
DO - 10.1007/s10529-012-1061-x
M3 - Journal article
AN - SCOPUS:84872801952
VL - 35
SP - 189
EP - 195
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 2
ER -
ID: 97187638