Oxalate found in wood cell wall during incipient brown rot degradation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Oxalate found in wood cell wall during incipient brown rot degradation. / Füchtner, Sophie; Alfredsen, Gry; Thygesen, Lisbeth G.

In: International Biodeterioration and Biodegradation, Vol. 177, 105531, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Füchtner, S, Alfredsen, G & Thygesen, LG 2023, 'Oxalate found in wood cell wall during incipient brown rot degradation', International Biodeterioration and Biodegradation, vol. 177, 105531. https://doi.org/10.1016/j.ibiod.2022.105531

APA

Füchtner, S., Alfredsen, G., & Thygesen, L. G. (2023). Oxalate found in wood cell wall during incipient brown rot degradation. International Biodeterioration and Biodegradation, 177, [105531]. https://doi.org/10.1016/j.ibiod.2022.105531

Vancouver

Füchtner S, Alfredsen G, Thygesen LG. Oxalate found in wood cell wall during incipient brown rot degradation. International Biodeterioration and Biodegradation. 2023;177. 105531. https://doi.org/10.1016/j.ibiod.2022.105531

Author

Füchtner, Sophie ; Alfredsen, Gry ; Thygesen, Lisbeth G. / Oxalate found in wood cell wall during incipient brown rot degradation. In: International Biodeterioration and Biodegradation. 2023 ; Vol. 177.

Bibtex

@article{9c7b921422974fb895d5e9c58f74d1e8,
title = "Oxalate found in wood cell wall during incipient brown rot degradation",
abstract = "Brown rot fungi are a marvel and an enigma of Nature. They are capable of depolymerizing holocellulose within wood cell walls without significantly mineralizing lignin. The exact details behind this feat remain unknown, but a staggered mechanism has been identified: 1) an initial step characterized by oxidative degradation of the wood cell wall biopolymers and hypothesized to involve transport of Fe3+ chelated by oxalate into the cell wall, and 2) a second degradation step dominated by hydrolytic enzymes, primarily endoglucanase activity. We subjected spruce wood (Picea abies) to Rhodonia placenta and isolated xylem tissue in the initial stage of degradation. Confocal Raman microscopy revealed oxalate accumulation in the secondary cell wall of a tracheid having fungal hyphae within the lumen. This observation is the first in situ verification of oxalate accumulation within the cell wall during the first step of brown rot degradation.",
keywords = "In situ localization, Picea abies, Raman microscopy, Rhodonia placenta",
author = "Sophie F{\"u}chtner and Gry Alfredsen and Thygesen, {Lisbeth G.}",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2023",
doi = "10.1016/j.ibiod.2022.105531",
language = "English",
volume = "177",
journal = "International Biodeterioration and Biodegradation",
issn = "0964-8305",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Oxalate found in wood cell wall during incipient brown rot degradation

AU - Füchtner, Sophie

AU - Alfredsen, Gry

AU - Thygesen, Lisbeth G.

N1 - Publisher Copyright: © 2022 The Authors

PY - 2023

Y1 - 2023

N2 - Brown rot fungi are a marvel and an enigma of Nature. They are capable of depolymerizing holocellulose within wood cell walls without significantly mineralizing lignin. The exact details behind this feat remain unknown, but a staggered mechanism has been identified: 1) an initial step characterized by oxidative degradation of the wood cell wall biopolymers and hypothesized to involve transport of Fe3+ chelated by oxalate into the cell wall, and 2) a second degradation step dominated by hydrolytic enzymes, primarily endoglucanase activity. We subjected spruce wood (Picea abies) to Rhodonia placenta and isolated xylem tissue in the initial stage of degradation. Confocal Raman microscopy revealed oxalate accumulation in the secondary cell wall of a tracheid having fungal hyphae within the lumen. This observation is the first in situ verification of oxalate accumulation within the cell wall during the first step of brown rot degradation.

AB - Brown rot fungi are a marvel and an enigma of Nature. They are capable of depolymerizing holocellulose within wood cell walls without significantly mineralizing lignin. The exact details behind this feat remain unknown, but a staggered mechanism has been identified: 1) an initial step characterized by oxidative degradation of the wood cell wall biopolymers and hypothesized to involve transport of Fe3+ chelated by oxalate into the cell wall, and 2) a second degradation step dominated by hydrolytic enzymes, primarily endoglucanase activity. We subjected spruce wood (Picea abies) to Rhodonia placenta and isolated xylem tissue in the initial stage of degradation. Confocal Raman microscopy revealed oxalate accumulation in the secondary cell wall of a tracheid having fungal hyphae within the lumen. This observation is the first in situ verification of oxalate accumulation within the cell wall during the first step of brown rot degradation.

KW - In situ localization

KW - Picea abies

KW - Raman microscopy

KW - Rhodonia placenta

U2 - 10.1016/j.ibiod.2022.105531

DO - 10.1016/j.ibiod.2022.105531

M3 - Journal article

AN - SCOPUS:85142199496

VL - 177

JO - International Biodeterioration and Biodegradation

JF - International Biodeterioration and Biodegradation

SN - 0964-8305

M1 - 105531

ER -

ID: 329622175