Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation

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Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation. / Dahl, Marius A; Areta, José Lisandro; Jeppesen, Per Bendix; Birk, Jesper Bratz; Johansen, Egil I; Ingemann-Hansen, Thorsten; Hansen, Mette; Skålhegg, Bjørn Steen; Ivy, John L; Wojtaszewski, Jørgen; Overgaard, Kristian; Jensen, Jørgen.

I: Journal of Applied Physiology, Bind 129, Nr. 2, 2020, s. 297-310.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Dahl, MA, Areta, JL, Jeppesen, PB, Birk, JB, Johansen, EI, Ingemann-Hansen, T, Hansen, M, Skålhegg, BS, Ivy, JL, Wojtaszewski, J, Overgaard, K & Jensen, J 2020, 'Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation', Journal of Applied Physiology, bind 129, nr. 2, s. 297-310. https://doi.org/10.1152/japplphysiol.00817.2019

APA

Dahl, M. A., Areta, J. L., Jeppesen, P. B., Birk, J. B., Johansen, E. I., Ingemann-Hansen, T., ... Jensen, J. (2020). Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation. Journal of Applied Physiology, 129(2), 297-310. https://doi.org/10.1152/japplphysiol.00817.2019

Vancouver

Dahl MA, Areta JL, Jeppesen PB, Birk JB, Johansen EI, Ingemann-Hansen T o.a. Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation. Journal of Applied Physiology. 2020;129(2):297-310. https://doi.org/10.1152/japplphysiol.00817.2019

Author

Dahl, Marius A ; Areta, José Lisandro ; Jeppesen, Per Bendix ; Birk, Jesper Bratz ; Johansen, Egil I ; Ingemann-Hansen, Thorsten ; Hansen, Mette ; Skålhegg, Bjørn Steen ; Ivy, John L ; Wojtaszewski, Jørgen ; Overgaard, Kristian ; Jensen, Jørgen. / Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation. I: Journal of Applied Physiology. 2020 ; Bind 129, Nr. 2. s. 297-310.

Bibtex

@article{7693083d40b34c8381e6ba7a24c45e36,
title = "Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation",
abstract = "The present study compared the effects of post exercise carbohydrate plus protein (CHO+PROT) and carbohydrate (CHO) only supplementation on muscle glycogen metabolism, anabolic cell signalling and subsequent exercise performance. Nine endurance-trained males cycled twice to exhaustion (muscle glycogen decreased from ~495 to ~125 mmol·kg dw-1) and received either CHO only (1.2 g·kg-1·h-1) or CHO+PROT (0.8/0.4 g·kg-1·h-1) during the first 90 min of recovery. Glycogen content was similar before the performance test after 5 h of recovery. Glycogen synthase (GS) fractional activity increased after exhaustive exercise and remained activated 5 h after despite substantial glycogen synthesis (176.1±19.1 and 204.6±27.0 mmol·kg dw-1 in CHO and CHO+PROT, respectively; p=0.15). Phosphorylation of GS at site 3 and site 2+2a remained low during recovery. After the 5 h recovery, cycling time to exhaustion was improved by CHO+PROT supplementation compared to CHO supplementation (54.6±11.0 vs 46.1±9.8 min; p=0.009). After the performance test, muscle glycogen was equally reduced in PRO+CHO and CHO. Akt Ser473 and p70s6k Thr389 phosphorylation was elevated after 5 h of recovery. There were no differences in Akt Ser473, p70s6k Thr389 or TSC2 Thr1462 phosphorylation between treatments. Nitrogen balance was positive in CHO+PROT (19.6±7.6 mg nitrogen·kg-1, p=0.04) and higher than CHO (-10.7±6.3 mg nitrogen·kg-1, p=0.009). Conclusion: CHO+PROT supplementation during exercise recovery improved subsequent endurance performance relative to consuming CHO only. This improved performance after CHO+PROT supplementation could not be accounted for by differences in glycogen metabolism or anabolic cell signaling, but may have been related to differences in nitrogen balance.",
keywords = "Faculty of Science, Exercise, Glycogen synthase, Akt/PKB, Protein synthesis, Nitrogen balance",
author = "Dahl, {Marius A} and Areta, {Jos{\'e} Lisandro} and Jeppesen, {Per Bendix} and Birk, {Jesper Bratz} and Johansen, {Egil I} and Thorsten Ingemann-Hansen and Mette Hansen and Sk{\aa}lhegg, {Bj{\o}rn Steen} and Ivy, {John L} and J{\o}rgen Wojtaszewski and Kristian Overgaard and J{\o}rgen Jensen",
note = "CURIS 2020 NEXS 258",
year = "2020",
doi = "10.1152/japplphysiol.00817.2019",
language = "English",
volume = "129",
pages = "297--310",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "2",

}

RIS

TY - JOUR

T1 - Coingestion of protein and carbohydrate in the early recovery phase, compared with carbohydrate only, improves endurance performance despite similar glycogen degradation and AMPK phosphorylation

AU - Dahl, Marius A

AU - Areta, José Lisandro

AU - Jeppesen, Per Bendix

AU - Birk, Jesper Bratz

AU - Johansen, Egil I

AU - Ingemann-Hansen, Thorsten

AU - Hansen, Mette

AU - Skålhegg, Bjørn Steen

AU - Ivy, John L

AU - Wojtaszewski, Jørgen

AU - Overgaard, Kristian

AU - Jensen, Jørgen

N1 - CURIS 2020 NEXS 258

PY - 2020

Y1 - 2020

N2 - The present study compared the effects of post exercise carbohydrate plus protein (CHO+PROT) and carbohydrate (CHO) only supplementation on muscle glycogen metabolism, anabolic cell signalling and subsequent exercise performance. Nine endurance-trained males cycled twice to exhaustion (muscle glycogen decreased from ~495 to ~125 mmol·kg dw-1) and received either CHO only (1.2 g·kg-1·h-1) or CHO+PROT (0.8/0.4 g·kg-1·h-1) during the first 90 min of recovery. Glycogen content was similar before the performance test after 5 h of recovery. Glycogen synthase (GS) fractional activity increased after exhaustive exercise and remained activated 5 h after despite substantial glycogen synthesis (176.1±19.1 and 204.6±27.0 mmol·kg dw-1 in CHO and CHO+PROT, respectively; p=0.15). Phosphorylation of GS at site 3 and site 2+2a remained low during recovery. After the 5 h recovery, cycling time to exhaustion was improved by CHO+PROT supplementation compared to CHO supplementation (54.6±11.0 vs 46.1±9.8 min; p=0.009). After the performance test, muscle glycogen was equally reduced in PRO+CHO and CHO. Akt Ser473 and p70s6k Thr389 phosphorylation was elevated after 5 h of recovery. There were no differences in Akt Ser473, p70s6k Thr389 or TSC2 Thr1462 phosphorylation between treatments. Nitrogen balance was positive in CHO+PROT (19.6±7.6 mg nitrogen·kg-1, p=0.04) and higher than CHO (-10.7±6.3 mg nitrogen·kg-1, p=0.009). Conclusion: CHO+PROT supplementation during exercise recovery improved subsequent endurance performance relative to consuming CHO only. This improved performance after CHO+PROT supplementation could not be accounted for by differences in glycogen metabolism or anabolic cell signaling, but may have been related to differences in nitrogen balance.

AB - The present study compared the effects of post exercise carbohydrate plus protein (CHO+PROT) and carbohydrate (CHO) only supplementation on muscle glycogen metabolism, anabolic cell signalling and subsequent exercise performance. Nine endurance-trained males cycled twice to exhaustion (muscle glycogen decreased from ~495 to ~125 mmol·kg dw-1) and received either CHO only (1.2 g·kg-1·h-1) or CHO+PROT (0.8/0.4 g·kg-1·h-1) during the first 90 min of recovery. Glycogen content was similar before the performance test after 5 h of recovery. Glycogen synthase (GS) fractional activity increased after exhaustive exercise and remained activated 5 h after despite substantial glycogen synthesis (176.1±19.1 and 204.6±27.0 mmol·kg dw-1 in CHO and CHO+PROT, respectively; p=0.15). Phosphorylation of GS at site 3 and site 2+2a remained low during recovery. After the 5 h recovery, cycling time to exhaustion was improved by CHO+PROT supplementation compared to CHO supplementation (54.6±11.0 vs 46.1±9.8 min; p=0.009). After the performance test, muscle glycogen was equally reduced in PRO+CHO and CHO. Akt Ser473 and p70s6k Thr389 phosphorylation was elevated after 5 h of recovery. There were no differences in Akt Ser473, p70s6k Thr389 or TSC2 Thr1462 phosphorylation between treatments. Nitrogen balance was positive in CHO+PROT (19.6±7.6 mg nitrogen·kg-1, p=0.04) and higher than CHO (-10.7±6.3 mg nitrogen·kg-1, p=0.009). Conclusion: CHO+PROT supplementation during exercise recovery improved subsequent endurance performance relative to consuming CHO only. This improved performance after CHO+PROT supplementation could not be accounted for by differences in glycogen metabolism or anabolic cell signaling, but may have been related to differences in nitrogen balance.

KW - Faculty of Science

KW - Exercise

KW - Glycogen synthase

KW - Akt/PKB

KW - Protein synthesis

KW - Nitrogen balance

U2 - 10.1152/japplphysiol.00817.2019

DO - 10.1152/japplphysiol.00817.2019

M3 - Journal article

C2 - 32584664

VL - 129

SP - 297

EP - 310

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 2

ER -

ID: 243852329