Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation

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Standard

Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation. / Knudsen, Jonas Roland; Steenberg, Dorte Enggaard; Hingst, Janne Rasmuss; Hodgson, Lorna R; Henriquez-Olguin, Carlos; Li, Zhencheng; Kiens, Bente; Richter, Erik A.; Wojtaszewski, Jørgen; Verkade, Paul; Jensen, Thomas Elbenhardt.

I: Molecular Metabolism, Bind 39, 100998, 2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Knudsen, JR, Steenberg, DE, Hingst, JR, Hodgson, LR, Henriquez-Olguin, C, Li, Z, Kiens, B, Richter, EA, Wojtaszewski, J, Verkade, P & Jensen, TE 2020, 'Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation', Molecular Metabolism, bind 39, 100998. https://doi.org/10.1016/j.molmet.2020.100998

APA

Knudsen, J. R., Steenberg, D. E., Hingst, J. R., Hodgson, L. R., Henriquez-Olguin, C., Li, Z., Kiens, B., Richter, E. A., Wojtaszewski, J., Verkade, P., & Jensen, T. E. (2020). Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation. Molecular Metabolism, 39, [100998]. https://doi.org/10.1016/j.molmet.2020.100998

Vancouver

Knudsen JR, Steenberg DE, Hingst JR, Hodgson LR, Henriquez-Olguin C, Li Z o.a. Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation. Molecular Metabolism. 2020;39. 100998. https://doi.org/10.1016/j.molmet.2020.100998

Author

Knudsen, Jonas Roland ; Steenberg, Dorte Enggaard ; Hingst, Janne Rasmuss ; Hodgson, Lorna R ; Henriquez-Olguin, Carlos ; Li, Zhencheng ; Kiens, Bente ; Richter, Erik A. ; Wojtaszewski, Jørgen ; Verkade, Paul ; Jensen, Thomas Elbenhardt. / Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation. I: Molecular Metabolism. 2020 ; Bind 39.

Bibtex

@article{7d8527c92ca447ed91d31587207e8c3e,
title = "Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation",
abstract = "Objective: Exercise is a cornerstone in the management of skeletal muscle insulin-resistance. A well-established benefit of a single bout of exercise is increased insulin sensitivity for hours post-exercise in the previously exercised musculature. Although rodent studies suggest that the insulin-sensitization phenomenon involves enhanced insulin-stimulated GLUT4 cell surface translocation and might involve intramuscular redistribution of GLUT4, the conservation to humans is unknown.Methods: Healthy young males underwent an insulin-sensitizing one-legged kicking exercise bout for 1 hour followed by fatigue bouts to exhaustion. Muscle biopsies were obtained 4h post-exercise before and after a 2h hyperinsulinemic-euglycemic clamp.Results: A detailed microscopy-based analysis of GLUT4 distribution muscle specimen in 7 different myocellular compartments revealed that prior exercise increased GLUT4 localization in insulin-responsive storage vesicles and T-tubuli. Furthermore, insulin-stimulated GLUT4 localization was augmented at the sarcolemma and in the endosomal compartments.Conclusion: An intracellular redistribution of GLUT4 post-exercise is proposed as a molecular mechanism contributing to the insulin-sensitizing effect of prior exercise in human skeletal muscle.",
keywords = "Faculty of Science, Exercise, Skeletal muscle, GLUT4, Insulin sensitivity, Insulin resistance",
author = "Knudsen, {Jonas Roland} and Steenberg, {Dorte Enggaard} and Hingst, {Janne Rasmuss} and Hodgson, {Lorna R} and Carlos Henriquez-Olguin and Zhencheng Li and Bente Kiens and Richter, {Erik A.} and J{\o}rgen Wojtaszewski and Paul Verkade and Jensen, {Thomas Elbenhardt}",
note = "Copyright {\textcopyright} 2020 The Author(s). Published by Elsevier GmbH.. All rights reserved.",
year = "2020",
doi = "10.1016/j.molmet.2020.100998",
language = "English",
volume = "39",
journal = "Molecular Metabolism",
issn = "2212-8778",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Prior exercise in humans redistributes intramuscular GLUT4 and enhances insulin-stimulated sarcolemmal and endosomal GLUT4 translocation

AU - Knudsen, Jonas Roland

AU - Steenberg, Dorte Enggaard

AU - Hingst, Janne Rasmuss

AU - Hodgson, Lorna R

AU - Henriquez-Olguin, Carlos

AU - Li, Zhencheng

AU - Kiens, Bente

AU - Richter, Erik A.

AU - Wojtaszewski, Jørgen

AU - Verkade, Paul

AU - Jensen, Thomas Elbenhardt

N1 - Copyright © 2020 The Author(s). Published by Elsevier GmbH.. All rights reserved.

PY - 2020

Y1 - 2020

N2 - Objective: Exercise is a cornerstone in the management of skeletal muscle insulin-resistance. A well-established benefit of a single bout of exercise is increased insulin sensitivity for hours post-exercise in the previously exercised musculature. Although rodent studies suggest that the insulin-sensitization phenomenon involves enhanced insulin-stimulated GLUT4 cell surface translocation and might involve intramuscular redistribution of GLUT4, the conservation to humans is unknown.Methods: Healthy young males underwent an insulin-sensitizing one-legged kicking exercise bout for 1 hour followed by fatigue bouts to exhaustion. Muscle biopsies were obtained 4h post-exercise before and after a 2h hyperinsulinemic-euglycemic clamp.Results: A detailed microscopy-based analysis of GLUT4 distribution muscle specimen in 7 different myocellular compartments revealed that prior exercise increased GLUT4 localization in insulin-responsive storage vesicles and T-tubuli. Furthermore, insulin-stimulated GLUT4 localization was augmented at the sarcolemma and in the endosomal compartments.Conclusion: An intracellular redistribution of GLUT4 post-exercise is proposed as a molecular mechanism contributing to the insulin-sensitizing effect of prior exercise in human skeletal muscle.

AB - Objective: Exercise is a cornerstone in the management of skeletal muscle insulin-resistance. A well-established benefit of a single bout of exercise is increased insulin sensitivity for hours post-exercise in the previously exercised musculature. Although rodent studies suggest that the insulin-sensitization phenomenon involves enhanced insulin-stimulated GLUT4 cell surface translocation and might involve intramuscular redistribution of GLUT4, the conservation to humans is unknown.Methods: Healthy young males underwent an insulin-sensitizing one-legged kicking exercise bout for 1 hour followed by fatigue bouts to exhaustion. Muscle biopsies were obtained 4h post-exercise before and after a 2h hyperinsulinemic-euglycemic clamp.Results: A detailed microscopy-based analysis of GLUT4 distribution muscle specimen in 7 different myocellular compartments revealed that prior exercise increased GLUT4 localization in insulin-responsive storage vesicles and T-tubuli. Furthermore, insulin-stimulated GLUT4 localization was augmented at the sarcolemma and in the endosomal compartments.Conclusion: An intracellular redistribution of GLUT4 post-exercise is proposed as a molecular mechanism contributing to the insulin-sensitizing effect of prior exercise in human skeletal muscle.

KW - Faculty of Science

KW - Exercise

KW - Skeletal muscle

KW - GLUT4

KW - Insulin sensitivity

KW - Insulin resistance

U2 - 10.1016/j.molmet.2020.100998

DO - 10.1016/j.molmet.2020.100998

M3 - Journal article

C2 - 32305516

VL - 39

JO - Molecular Metabolism

JF - Molecular Metabolism

SN - 2212-8778

M1 - 100998

ER -

ID: 241043065