Oxygen Constraints on Microbial Secretomes during
Plant Cell Wall Turnover

The OxyMiST consortium will establish how oxygen contributes to whole plant cell wall (PCW) degradation by microbial secretomes. The role of oxygen in promoting and restricting these enzymatic processes has been largely overlooked in the past.

The project extends from environmental microbial diversity to metasecretomics, from controlled laboratory PCW degradation to defined enzyme reactivity, and from molecular structure to fine-tuned enzymatic modifications. Leading scientists in Copenhagen, Marseille and Cambridge will take advantage of existing expertise and infrastructure to develop new advanced methodologies such as phylopeptidomics for this study.

The primary outcome of this research will be a completely revised view of the dynamic and temporal changes in the spectrum and activity of enzymes secreted during the turnover of PCWs. In particular, the constraint imparted by the dissolved O2 in two ecosystems (peat and wood) will be defined.



Methionine oxidation of carbohydrate-active enzymes during white-rot wood decay
Lise Molinelli, Jean-Guy Berrin, Lionel Tarrago, et al. (2024), Appl Environ Microbiol 90:e01931-23

At the root of plant symbioses: Untangling the genetic mechanisms behind mutualistic associations
Annie Lebreton, Jean Keller  (2024), Curr Opin Plant Biol 77:102448

Stable functional structure despite high taxonomic variability across fungal communities in soils of old-growth montane forests
Qingchao Zeng, Annie Lebreton, Francis M. Martin, et al.  (2023), Microbiome 11, 217

The Ustilago maydis AA10 LPMO is active on fungal cell wall chitin
Roseline A. Yao, Jean-Guy Berrin, et al. (2023), Appl Environ Microbiol 89:e00573-23

A fast, sensitive and fluorescent LPMO activity assay
Johan Ø. Ipsen, Katja S. Johansen, Søren Brander (2023), Front Microbiol 14:1128470

Eudicot primary cell wall glucomannan is related in synthesis, structure, and function to xyloglucan
Li Yu, Paul Dupree, et al. (2022), The Plant Cell 34, 4600-4622


Professor Francis Martin
INRAE, Nancy, France

Senior Science Manager Kristian B. Krogh
Novozymes A/S

Professor Henrik Scheller
Berkley University of California




Name Title Phone E-mail
Anne Blicher Laboratory Technician +4535328028 E-mail
Helle Jakobe Martens Associate Professor +4528960218 E-mail
Jonas Thomsen PhD Fellow   E-mail
Magnus Hallas-Møller Assistant Professor +4535332313 E-mail
Radina Tokin Postdoc +4535326455 E-mail
Signe Lett Assistant Professor +4535326162 E-mail
Søren Brander Associate Professor +4535331261 E-mail

External members  - University of Cambridge:

Name Title Phone E-mail
Paul Dupree PI, Professor E-mail
Theodora Tryfona Postdoc E-mail
Darragh Kelleher PhD student E-mail
Xiao-Lan Yu Technical Scientist E-mail

External members - NRAE:

Name Title Phone E-mail
Jean-Guy Berrin PI, Senior research scientist E-mail
Marie-Noëlle Rosso Research scientist E-mail
Lionel Tarrago Research scientist E-mail
Bernard Henrissat Professor E-mail
Nicolas Terrapon Assistant Professor E-mail
Robert Röllig Postdoc E-mail
Annie Lebreton Postdoc E-mail
Thiago Augusto Gonçalves Postdoc E-mail

Funded by:

Novo Nordisk Foundation

The OxyMiST project is funded by a six year grant from the Novo Nordisk Foundation, Grant number NNF20OC0059697

Project: Oxygen Constraints on Microbial Secretomes during Plant Cell Wall Turnover (OxyMiST)
Period: Apr. 2021 – Mar. 2028


Grant holder
Søren Brander
Tel: +45 3533 1261
E-mail: sbd@ign.ku.dk

Project administrator
Radina Tokin
Tel: +45 3532 6455
E-mail: rato@ign.ku.dk

Grant recipient
Prof. Katja S. Johansen
E-mail: ksj36@cam.ac.uk