Advances in the analysis of biogeochemical interfaces. Nanosims to investigate soil microenvironments
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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Advances in the analysis of biogeochemical interfaces. Nanosims to investigate soil microenvironments. / Mueller, Carsten W.; Weber, Peter K.; Kilburn, Matt R.; Hoeschen, Carmen; Kleber, Markus; Pett-Ridge, Jennifer.
Advances in Agronomy. Academic Press, 2013. p. 1-46 (Advances in Agronomy, Vol. 121).Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
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TY - CHAP
T1 - Advances in the analysis of biogeochemical interfaces. Nanosims to investigate soil microenvironments
AU - Mueller, Carsten W.
AU - Weber, Peter K.
AU - Kilburn, Matt R.
AU - Hoeschen, Carmen
AU - Kleber, Markus
AU - Pett-Ridge, Jennifer
PY - 2013
Y1 - 2013
N2 - Since a NanoSIMS high-resolution secondary ion mass spectrometry (SIMS) instrument was first used for cosmochemistry investigations over a decade ago, both interest in NanoSIMS and the number of instruments available have significantly increased. However, SIMS comes with a set of challenges that are of both technical and conceptual nature, particularly for complex samples such as soils. Here, we synthesize existing research and provide conceptual and technical guidance to those who wish to investigate soil processes at the submicron scale using SIMS, specifically with NanoSIMS. Our review not only offers advice resulting from our own operational experience but also intends to promote synergistic research on yet unresolved methodological issues. We identify and describe the basic setup of a NanoSIMS instrument, and important issues that may arise as a soil sample specimen are prepared for NanoSIMS analysis. This is complemented by discussions of experimental design, data analysis, and data representation. Next to experimental design, sample preparation is the most crucial prerequisite for successful NanoSIMS analyses. We discuss the requirements and limitations for sample preparation over the size range from individual soil particles to intact soil structures such as macroaggregates or intact soil cores. For robust interpretation of data obtained by NanoSIMS, parallel spatial, textural (scanning electron microscopy, atomic force microscopy), or compositional analyses (scanning transmission X-ray microscopy) are often necessary to provide necessary context. We suggest that NanoSIMS analysis is most valuable when applied in concert with other analytical procedures and can provide powerful inference about small-scale processes that can be traced via isotopic labeling or elemental mapping.
AB - Since a NanoSIMS high-resolution secondary ion mass spectrometry (SIMS) instrument was first used for cosmochemistry investigations over a decade ago, both interest in NanoSIMS and the number of instruments available have significantly increased. However, SIMS comes with a set of challenges that are of both technical and conceptual nature, particularly for complex samples such as soils. Here, we synthesize existing research and provide conceptual and technical guidance to those who wish to investigate soil processes at the submicron scale using SIMS, specifically with NanoSIMS. Our review not only offers advice resulting from our own operational experience but also intends to promote synergistic research on yet unresolved methodological issues. We identify and describe the basic setup of a NanoSIMS instrument, and important issues that may arise as a soil sample specimen are prepared for NanoSIMS analysis. This is complemented by discussions of experimental design, data analysis, and data representation. Next to experimental design, sample preparation is the most crucial prerequisite for successful NanoSIMS analyses. We discuss the requirements and limitations for sample preparation over the size range from individual soil particles to intact soil structures such as macroaggregates or intact soil cores. For robust interpretation of data obtained by NanoSIMS, parallel spatial, textural (scanning electron microscopy, atomic force microscopy), or compositional analyses (scanning transmission X-ray microscopy) are often necessary to provide necessary context. We suggest that NanoSIMS analysis is most valuable when applied in concert with other analytical procedures and can provide powerful inference about small-scale processes that can be traced via isotopic labeling or elemental mapping.
KW - Fluorescent in situ hybridization
KW - Isotopic enrichment
KW - Microaggregates
KW - Near edge X-ray absorption fine structure spectrometry
KW - Organo-mineral associations
KW - Rhizosphere
KW - Scanning electron microscopy
KW - Scanning transmission X-ray microscopy
KW - Secondary ion mass spectrometry
KW - Transmission electron microscopy
U2 - 10.1016/B978-0-12-407685-3.00001-3
DO - 10.1016/B978-0-12-407685-3.00001-3
M3 - Book chapter
AN - SCOPUS:84879257042
T3 - Advances in Agronomy
SP - 1
EP - 46
BT - Advances in Agronomy
PB - Academic Press
ER -
ID: 239162046