Stable-isotope Raman microspectroscopy for the analysis of soil organic matter
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Stable-isotope Raman microspectroscopy for the analysis of soil organic matter. / Wiesheu, Alexandra C.; Brejcha, Ramona; Mueller, Carsten W.; Kögel-Knabner, Ingrid; Elsner, Martin; Niessner, Reinhard; Ivleva, Natalia P.
In: Analytical and Bioanalytical Chemistry, Vol. 410, No. 3, 2018, p. 923-931.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Stable-isotope Raman microspectroscopy for the analysis of soil organic matter
AU - Wiesheu, Alexandra C.
AU - Brejcha, Ramona
AU - Mueller, Carsten W.
AU - Kögel-Knabner, Ingrid
AU - Elsner, Martin
AU - Niessner, Reinhard
AU - Ivleva, Natalia P.
PY - 2018
Y1 - 2018
N2 - We examined the potential of stable-isotope Raman microspectroscopy (SIRM) for the evaluation of differently enriched 13C-labeled humic acids as model substances for soil organic matter (SOM). The SOM itself can be linked to the soil water holding capacity. Therefore, artificial humic acids (HA) with known isotopic compositions were synthesized and analyzed by means of SIRM. By performing a pregraphitization, a suitable analysis method was developed to cope with the high fluorescence background. Results were verified against isotope ratio mass spectrometry (IRMS). The limit of quantification was 2.1 × 10−1 13C/Ctot for the total region and 3.2 × 10−2 13C/Ctot for a linear correlation up to 0.25 13C/Ctot. Complementary nanoscale secondary ion mass spectrometry (NanoSIMS) analysis indicated small-scale heterogeneity within the dry sample material, even though—owing to sample topography and occurring matrix effects—obtained values deviated in magnitude from those of IRMS and SIRM. Our study shows that SIRM is well-suited for the analysis of stable isotope-labeled HA. This method requires no specific sample preparation and can provide information with a spatial resolution in the micrometer range. [Figure not available: see fulltext.].
AB - We examined the potential of stable-isotope Raman microspectroscopy (SIRM) for the evaluation of differently enriched 13C-labeled humic acids as model substances for soil organic matter (SOM). The SOM itself can be linked to the soil water holding capacity. Therefore, artificial humic acids (HA) with known isotopic compositions were synthesized and analyzed by means of SIRM. By performing a pregraphitization, a suitable analysis method was developed to cope with the high fluorescence background. Results were verified against isotope ratio mass spectrometry (IRMS). The limit of quantification was 2.1 × 10−1 13C/Ctot for the total region and 3.2 × 10−2 13C/Ctot for a linear correlation up to 0.25 13C/Ctot. Complementary nanoscale secondary ion mass spectrometry (NanoSIMS) analysis indicated small-scale heterogeneity within the dry sample material, even though—owing to sample topography and occurring matrix effects—obtained values deviated in magnitude from those of IRMS and SIRM. Our study shows that SIRM is well-suited for the analysis of stable isotope-labeled HA. This method requires no specific sample preparation and can provide information with a spatial resolution in the micrometer range. [Figure not available: see fulltext.].
KW - Humic acids
KW - Raman microspectroscopy
KW - Soil organic matter
KW - Stable isotopes
U2 - 10.1007/s00216-017-0543-z
DO - 10.1007/s00216-017-0543-z
M3 - Journal article
C2 - 28808741
AN - SCOPUS:85028554613
VL - 410
SP - 923
EP - 931
JO - Analusis
JF - Analusis
SN - 0365-4877
IS - 3
ER -
ID: 238953460