Visualisation of morphological interactionof diamond and silver nanoparticles with Salmonella enteritidis and Listeria Monocytogenes
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Visualisation of morphological interactionof diamond and silver nanoparticles with Salmonella enteritidis and Listeria Monocytogenes. / Sawosz, Ewa; Chwalibog, André; Mitura, Katarzyna; Mitura, Stanislaw; Szeliga, Jacek; Niemiec, Tomasz; Rupiewicz, Marlena; Grodzik, Marta; Sokolowska, Aleksandra.
In: Journal of Nanoscience and Nanotechnology, Vol. 11, No. 9, 2011, p. 7635-7641.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Visualisation of morphological interactionof diamond and silver nanoparticles with Salmonella enteritidis and Listeria Monocytogenes
AU - Sawosz, Ewa
AU - Chwalibog, André
AU - Mitura, Katarzyna
AU - Mitura, Stanislaw
AU - Szeliga, Jacek
AU - Niemiec, Tomasz
AU - Rupiewicz, Marlena
AU - Grodzik, Marta
AU - Sokolowska, Aleksandra
PY - 2011
Y1 - 2011
N2 - Currently, medicine intensively searches for methods to transport drugs to a target (sick) point within the body. The objective of the present investigation was to evaluate morphological characteristics of the assembles of silver or diamond nanoparticles with Salmonella Enteritidis (G-) or Listeria monocytogenes (G+), to reveal possibilities of constructing nanoparticle-bacteria vehicles. Diamond nanoparticles (nano-D) were produced by the detonation method. Hydrocolloids of silver nanoparticles (nano-Ag) were produced by electric non-explosive patented method. Hydrocolloids of nanoparticles (200 microl) were added to bacteria suspension (200 microl) in the following order: nano-D + Salmonella E.; nano-D + Listeria monocytogenes; nano-Ag + Salmonella E; nano-Ag + Listeria monocytogenes. Samples were inspected by transmission electron microscopy. Visualisation of nanoparticles and bacteria interaction showed harmful effects of both nanoparticles on bacteria morphology. The most spectacular effect of nano-D were strong links between nano-D packages and the flagella of Salmonella E. Nano-Ag were closely attached to Listeria monocytogenes but not to Salmonella E. There was no evidence of entering nano-Ag inside Listeria monocytogenes but smaller particles were placed inside Salmonella E. The ability of nano-D to attach to the flagella and the ability of nano-Ag to penetrate inside bacteria cells can be utilized to design nano-bacteria vehicles, being carriers for active substances attached to nanoparticles.
AB - Currently, medicine intensively searches for methods to transport drugs to a target (sick) point within the body. The objective of the present investigation was to evaluate morphological characteristics of the assembles of silver or diamond nanoparticles with Salmonella Enteritidis (G-) or Listeria monocytogenes (G+), to reveal possibilities of constructing nanoparticle-bacteria vehicles. Diamond nanoparticles (nano-D) were produced by the detonation method. Hydrocolloids of silver nanoparticles (nano-Ag) were produced by electric non-explosive patented method. Hydrocolloids of nanoparticles (200 microl) were added to bacteria suspension (200 microl) in the following order: nano-D + Salmonella E.; nano-D + Listeria monocytogenes; nano-Ag + Salmonella E; nano-Ag + Listeria monocytogenes. Samples were inspected by transmission electron microscopy. Visualisation of nanoparticles and bacteria interaction showed harmful effects of both nanoparticles on bacteria morphology. The most spectacular effect of nano-D were strong links between nano-D packages and the flagella of Salmonella E. Nano-Ag were closely attached to Listeria monocytogenes but not to Salmonella E. There was no evidence of entering nano-Ag inside Listeria monocytogenes but smaller particles were placed inside Salmonella E. The ability of nano-D to attach to the flagella and the ability of nano-Ag to penetrate inside bacteria cells can be utilized to design nano-bacteria vehicles, being carriers for active substances attached to nanoparticles.
KW - Former LIFE faculty
KW - Nanoparticles
KW - Dimond
KW - Silver
KW - Bacteria
KW - Morphlogy
KW - Drug Delivry
M3 - Journal article
VL - 11
SP - 7635
EP - 7641
JO - Journal of Nanoscience and Nanotechnology
JF - Journal of Nanoscience and Nanotechnology
SN - 1533-4880
IS - 9
ER -
ID: 35084888