Development of elite indica rice lines with wide spectrum of resistance to Thai blast isolates by pyramiding multiple resistance QTLs
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
With 1 figure and 2 tablesThe rice varieties IR64 and Jao Hom Nin (JHN) demonstrated a broad-spectrum resistance against the rice blast pathogens in Thailand. A genomic investigation unravelled many resistance genes residing on four genomic regions, chromosome 2 and 12 in IR64 and 1 and 11 in JHN. A cross between these varieties was made to combine resistance genes into a single genotype. Marker-assisted selection (MAS) was employed to identify F2 and F3 plants carrying a combination of four resistance QTLs in a homozygous fusion. Flanking markers RM212/RM319 and RM144/RM139 to blast resistant QTLs on chromosome 1 and 11 in JHN rice and tightly-linked markers RM208 and RM179 to blast resistant QTLs on chromosome 2 and 12 in IR64 rice variety were used for MAS. The stepwise MAS screening was brought in as a strategy to provide a cost-saving and minimum number of PCR performing to select resistant genotypes. F4 generation, lines carrying all resistant QTLs show a broader spectrum of resistance against 11 representatives of Thai blast pathogen isolates.
Originalsprog | Engelsk |
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Tidsskrift | Plant Breeding |
Vol/bind | 129 |
Udgave nummer | 2 |
Sider (fra-til) | 176-180 |
Antal sider | 5 |
ISSN | 0179-9541 |
DOI | |
Status | Udgivet - apr. 2010 |
ID: 291619656