OxyMiST
Oxygen Constraints on Microbial Secretomes during
Plant Cell Wall Turnover
The project extends from environmental microbial diversity to metasecretomics, from controlled laboratory PCW degradation to defined enzyme reactivity, and from molecular structure to fine-tuned enzymatic modifications. Leading scientists in Copenhagen, Marseille and Cambridge will take advantage of existing expertise and infrastructure to develop new advanced methodologies such as phylopeptidomics for this study.
The primary outcome of this research will be a completely revised view of the dynamic and temporal changes in the spectrum and activity of enzymes secreted during the turnover of PCWs. In particular, the constraint imparted by the dissolved O2 in two ecosystems (peat and wood) will be defined.
The project will integrate research carried out at the three research institutions:
- French National Institute for Agricultural Research, Food and Environment – INRAE, Marseille
- The University of Cambridge - UC, Cambridge
- The University of Copenhagen - UCPH, Copenhagen.
The work is organised in three broad themes encompassing ecology, microbiology and enzymology.
Methionine oxidation of carbohydrate-active enzymes during white-rot wood decay
Lise Molinelli, Jean-Guy Berrin, Lionel Tarrago, et al. (2024), Appl Environ Microbiol 90:e01931-23
At the root of plant symbioses: Untangling the genetic mechanisms behind mutualistic associations
Annie Lebreton, Jean Keller (2024), Curr Opin Plant Biol 77:102448
Stable functional structure despite high taxonomic variability across fungal communities in soils of old-growth montane forests
Qingchao Zeng, Annie Lebreton, Francis M. Martin, et al. (2023), Microbiome 11, 217
The Ustilago maydis AA10 LPMO is active on fungal cell wall chitin
Roseline A. Yao, Jean-Guy Berrin, et al. (2023), Appl Environ Microbiol 89:e00573-23
A fast, sensitive and fluorescent LPMO activity assay
Johan Ø. Ipsen, Katja S. Johansen, Søren Brander (2023), Front Microbiol 14:1128470
Eudicot primary cell wall glucomannan is related in synthesis, structure, and function to xyloglucan
Li Yu, Paul Dupree, et al. (2022), The Plant Cell 34, 4600-4622
Professor Francis Martin
INRAE, Nancy, France
Senior Science Manager Kristian B. Krogh
Novozymes A/S
Professor Henrik Scheller
Berkley University of California
Researchers
| Name | Title | Phone | |
|---|---|---|---|
| Anne Blicher | Laboratory Technician | +4535328028 | |
| Helle Jakobe Martens | Associate Professor | ||
| Magnus Hallas-Møller | Assistant Professor | +4535332313 | |
| Radina Tokin | Postdoc | +4535326455 | |
| Signe Lett | Assistant Professor | +4535326162 | |
| Søren Brander | Associate Professor | +4535331261 |
External members - University of Cambridge:
| Name | Title | Phone | |
|---|---|---|---|
| Paul Dupree | PI, Professor | ||
| Theodora Tryfona | Postdoc | ||
| Tomohiro Kuga | Postdoc | ||
| Darragh Kelleher | PhD student | ||
| Xiao-Lan Yu | Technical Scientist |
External members - NRAE:
| Name | Title | Phone | |
|---|---|---|---|
| Jean-Guy Berrin | PI, Senior research scientist | ||
| Marie-Noëlle Rosso | Research scientist | ||
| Lionel Tarrago | Research scientist | ||
| Bernard Henrissat | Professor | ||
| Thiago Augusto Gonçalves | Postdoc | ||
| Lucia Bidondo | PhD student |
Funded by:
The OxyMiST project is funded by the Novo Nordisk Foundation, Grant number NNF20OC0059697
Project: Oxygen Constraints on Microbial Secretomes during Plant Cell Wall Turnover (OxyMiST)
Period: Apr. 2021 – Mar. 2028
Contact
Grant holder
Søren Brander
Tel: +45 3533 1261
E-mail: sbd@ign.ku.dk
Project administrator
Radina Tokin
Tel: +45 3532 6455
E-mail: rato@ign.ku.dk
Grant recipient
Prof. Katja S. Johansen
E-mail: ksj36@cam.ac.uk
