Oxalate found in wood cell wall during incipient brown rot degradation
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Oxalate found in wood cell wall during incipient brown rot degradation. / Füchtner, Sophie; Alfredsen, Gry; Thygesen, Lisbeth G.
In: International Biodeterioration and Biodegradation, Vol. 177, 105531, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Oxalate found in wood cell wall during incipient brown rot degradation
AU - Füchtner, Sophie
AU - Alfredsen, Gry
AU - Thygesen, Lisbeth G.
N1 - Publisher Copyright: © 2022 The Authors
PY - 2023
Y1 - 2023
N2 - Brown rot fungi are a marvel and an enigma of Nature. They are capable of depolymerizing holocellulose within wood cell walls without significantly mineralizing lignin. The exact details behind this feat remain unknown, but a staggered mechanism has been identified: 1) an initial step characterized by oxidative degradation of the wood cell wall biopolymers and hypothesized to involve transport of Fe3+ chelated by oxalate into the cell wall, and 2) a second degradation step dominated by hydrolytic enzymes, primarily endoglucanase activity. We subjected spruce wood (Picea abies) to Rhodonia placenta and isolated xylem tissue in the initial stage of degradation. Confocal Raman microscopy revealed oxalate accumulation in the secondary cell wall of a tracheid having fungal hyphae within the lumen. This observation is the first in situ verification of oxalate accumulation within the cell wall during the first step of brown rot degradation.
AB - Brown rot fungi are a marvel and an enigma of Nature. They are capable of depolymerizing holocellulose within wood cell walls without significantly mineralizing lignin. The exact details behind this feat remain unknown, but a staggered mechanism has been identified: 1) an initial step characterized by oxidative degradation of the wood cell wall biopolymers and hypothesized to involve transport of Fe3+ chelated by oxalate into the cell wall, and 2) a second degradation step dominated by hydrolytic enzymes, primarily endoglucanase activity. We subjected spruce wood (Picea abies) to Rhodonia placenta and isolated xylem tissue in the initial stage of degradation. Confocal Raman microscopy revealed oxalate accumulation in the secondary cell wall of a tracheid having fungal hyphae within the lumen. This observation is the first in situ verification of oxalate accumulation within the cell wall during the first step of brown rot degradation.
KW - In situ localization
KW - Picea abies
KW - Raman microscopy
KW - Rhodonia placenta
U2 - 10.1016/j.ibiod.2022.105531
DO - 10.1016/j.ibiod.2022.105531
M3 - Journal article
AN - SCOPUS:85142199496
VL - 177
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
SN - 0964-8305
M1 - 105531
ER -
ID: 329622175