A randomised, controlled, crossover study of the effect of diet on angiopoietin-like protein 4 (ANGPTL4) through modification of the gut microbiome
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A randomised, controlled, crossover study of the effect of diet on angiopoietin-like protein 4 (ANGPTL4) through modification of the gut microbiome. / Blædel, Trine; Holm, Jacob Bak; Sundekilde, Ulrik K.; Schmedes, Mette S.; Hess, Anne Lundby; Lorenzen, Janne Kunchel; Kristiansen, Karsten; Dalsgaard, Trine K.; Astrup, Arne; Larsen, Lesli Hingstrup.
In: Journal of Nutritional Science, Vol. 5, e45, 2016.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A randomised, controlled, crossover study of the effect of diet on angiopoietin-like protein 4 (ANGPTL4) through modification of the gut microbiome
AU - Blædel, Trine
AU - Holm, Jacob Bak
AU - Sundekilde, Ulrik K.
AU - Schmedes, Mette S.
AU - Hess, Anne Lundby
AU - Lorenzen, Janne Kunchel
AU - Kristiansen, Karsten
AU - Dalsgaard, Trine K.
AU - Astrup, Arne
AU - Larsen, Lesli Hingstrup
N1 - CURIS 2016 NEXS 389
PY - 2016
Y1 - 2016
N2 - Angiopoietin-like protein 4 (ANGPTL4) is a lipoprotein lipase inhibitor that is involved in lipid metabolism and angiogenesis. Animal studies have suggestedthat the ANGPTL4 protein is modulated by the gut microbiota, possibly through increased concentrations of SCFA, such as C4, found in whole-fat milk or as a result of fermentation of inulin. This study investigated whether a standardised diet either high in fat content or supplemented with inulin powder would increase plasma ANGPTL4 in overweight men and whether this increase was mediated through a compositional change of the gut microbiota. The study had a crossover design with three arms, where participants were given a standardised isoenergetic diet supplemented with inulin powder, whole-fat milk or water (control). Plasma and urine samples were collected before and after each intervention period. Faecal samples and adipose tissue biopsies were collected after each intervention period. The study included twenty-one participants of whom eighteen completed the study. The dietary interventions did not change ANGPTL4 plasma concentration, nor was plasma ANGPTL4 associated with plasma lipids, TAG or NEFA concentration. The relative abundance of bifidobacteria following the inulin diet was higher, compared with the control diet. However, the changes in microbiota were not associated with plasma ANGPTL4 and the overall composition of the microbiota did not change between the dietary periods. Although weight was maintained throughout the dietary periods, weight was negatively associated with plasma ANGPTL4 concentration. In the adipose tissue, ANGPTL4 expression was correlated with leptin expression, but not with hypoxia-inducible factor 1α (HIF-1α) expression.
AB - Angiopoietin-like protein 4 (ANGPTL4) is a lipoprotein lipase inhibitor that is involved in lipid metabolism and angiogenesis. Animal studies have suggestedthat the ANGPTL4 protein is modulated by the gut microbiota, possibly through increased concentrations of SCFA, such as C4, found in whole-fat milk or as a result of fermentation of inulin. This study investigated whether a standardised diet either high in fat content or supplemented with inulin powder would increase plasma ANGPTL4 in overweight men and whether this increase was mediated through a compositional change of the gut microbiota. The study had a crossover design with three arms, where participants were given a standardised isoenergetic diet supplemented with inulin powder, whole-fat milk or water (control). Plasma and urine samples were collected before and after each intervention period. Faecal samples and adipose tissue biopsies were collected after each intervention period. The study included twenty-one participants of whom eighteen completed the study. The dietary interventions did not change ANGPTL4 plasma concentration, nor was plasma ANGPTL4 associated with plasma lipids, TAG or NEFA concentration. The relative abundance of bifidobacteria following the inulin diet was higher, compared with the control diet. However, the changes in microbiota were not associated with plasma ANGPTL4 and the overall composition of the microbiota did not change between the dietary periods. Although weight was maintained throughout the dietary periods, weight was negatively associated with plasma ANGPTL4 concentration. In the adipose tissue, ANGPTL4 expression was correlated with leptin expression, but not with hypoxia-inducible factor 1α (HIF-1α) expression.
KW - Faculty of Science
KW - Angiopoietin-like protein 4
KW - Lipid metabolism
KW - Lipoprotein lipase
KW - Obesity
KW - Gut microbiome
U2 - 10.1017/jns.2016.38
DO - 10.1017/jns.2016.38
M3 - Journal article
C2 - 28620472
VL - 5
JO - Journal of Nutritional Science
JF - Journal of Nutritional Science
SN - 2048-6790
M1 - e45
ER -
ID: 174013961