Danish apple cultivars: genetic diversity, parentage and breeding potential
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Danish apple cultivars : genetic diversity, parentage and breeding potential. / Larsen, Bjarne; Pedersen, Carsten; Ørgaard, Marian; Toldam-Andersen, Torben.
Proceedings of the XIV EUCARPIA Symposium on Fruit Breeding and Genetics . International Society for Horticultural Science, 2017. p. 355-358 (Acta Horticulturae, Vol. 1172).Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review
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TY - GEN
T1 - Danish apple cultivars
AU - Larsen, Bjarne
AU - Pedersen, Carsten
AU - Ørgaard, Marian
AU - Toldam-Andersen, Torben
N1 - Conference code: 14
PY - 2017
Y1 - 2017
N2 - AbstractWe made a genetic analysis of about 500 apple cultivars, which either originatefrom Denmark or have been of greater importance in Denmark. To study the diversity and parentage among the cultivars, 15 SSR markers were used. A new PCR-based protocol for identification of S-alleles was developed and used to study the S-alleles among cultivars. The protocol using general S-locus primers and digestion with two restriction enzymes, for fragment analysis on an automated DNA sequencer fastens the process and gives an accurate estimation of fragment lengths. The ploidy level was determined using flow-cytometry. All cultivars were distinguishable by the SSRmarkers though Danish originating cultivars did not form a group distinct from foreign cultivars. The parentage of several well-known cultivars was revealed.
AB - AbstractWe made a genetic analysis of about 500 apple cultivars, which either originatefrom Denmark or have been of greater importance in Denmark. To study the diversity and parentage among the cultivars, 15 SSR markers were used. A new PCR-based protocol for identification of S-alleles was developed and used to study the S-alleles among cultivars. The protocol using general S-locus primers and digestion with two restriction enzymes, for fragment analysis on an automated DNA sequencer fastens the process and gives an accurate estimation of fragment lengths. The ploidy level was determined using flow-cytometry. All cultivars were distinguishable by the SSRmarkers though Danish originating cultivars did not form a group distinct from foreign cultivars. The parentage of several well-known cultivars was revealed.
KW - Apples
KW - Genetisk diversitet
KW - Forædlingspotentiale
U2 - 10.17660/ActaHortic.2017.1172.66
DO - 10.17660/ActaHortic.2017.1172.66
M3 - Article in proceedings
T3 - Acta Horticulturae
SP - 355
EP - 358
BT - Proceedings of the XIV EUCARPIA Symposium on Fruit Breeding and Genetics
PB - International Society for Horticultural Science
Y2 - 14 June 2015 through 18 June 2015
ER -
ID: 186529046