Canine specific ELISA for coagulation factor VII
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Canine specific ELISA for coagulation factor VII. / Knudsen, Tom; Kjelgaard-Hansen, Mads; Tranholm, Mikael; Wiinberg, Bo; Clausen, Jes T.; Hansen, Jens Jacob; Nichols, Timothy C.; Kjalke, Marianne; Jensen, Asger Lundorff; Kristensen, Annemarie Thuri.
In: The Veterinary Journal, Vol. 190, No. 3, 2011, p. 352-358.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Canine specific ELISA for coagulation factor VII
AU - Knudsen, Tom
AU - Kjelgaard-Hansen, Mads
AU - Tranholm, Mikael
AU - Wiinberg, Bo
AU - Clausen, Jes T.
AU - Hansen, Jens Jacob
AU - Nichols, Timothy C.
AU - Kjalke, Marianne
AU - Jensen, Asger Lundorff
AU - Kristensen, Annemarie Thuri
PY - 2011
Y1 - 2011
N2 - Canine coagulation factor VII (FVII) deficiency can be hereditary or acquired and may cause life threatening bleeding episodes if untreated. FVII procoagulant activity can be measured by FVII activity (FVII:C), but assays for measurement of canine specific FVII antigen (FVII:Ag) have not been available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable in the diagnosis of hereditary canine FVII deficiency.
AB - Canine coagulation factor VII (FVII) deficiency can be hereditary or acquired and may cause life threatening bleeding episodes if untreated. FVII procoagulant activity can be measured by FVII activity (FVII:C), but assays for measurement of canine specific FVII antigen (FVII:Ag) have not been available to date. In this study, a canine specific ELISA for measurement of FVII:Ag in plasma was developed and validated. The FVII:Ag ELISA correctly diagnosed homozygous and heterozygous hereditary FVII deficiency. Together with activity based assays, such as FVII:C, the FVII:Ag ELISA should be valuable in the diagnosis of hereditary canine FVII deficiency.
KW - Former LIFE faculty
KW - FVII deficiency
KW - Haemostasis
KW - Coagulation factor
KW - ELISA
KW - Plasma
KW - Immunoassays
U2 - 10.1016/j.tvjl.2010.11.010
DO - 10.1016/j.tvjl.2010.11.010
M3 - Journal article
VL - 190
SP - 352
EP - 358
JO - The Veterinary Journal
JF - The Veterinary Journal
SN - 1090-0233
IS - 3
ER -
ID: 35352398