Cell differentiation and mycorrhizal infection in Dactylorhiza majalis (Rchb. f.) Hunt & Summerh. (Orchidaceae) during germination in vitro
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Cell differentiation and mycorrhizal infection in Dactylorhiza majalis (Rchb. f.) Hunt & Summerh. (Orchidaceae) during germination in vitro. / RASMUSSEN, HANNE N.
In: New Phytologist, Vol. 116, No. 1, 01.01.1990, p. 137-147.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cell differentiation and mycorrhizal infection in Dactylorhiza majalis (Rchb. f.) Hunt & Summerh. (Orchidaceae) during germination in vitro
AU - RASMUSSEN, HANNE N.
PY - 1990/1/1
Y1 - 1990/1/1
N2 - Seeds of Dactylorhiza majalis (Rchb. f.) Hunt & Summerhayes were sown in vitro with a compatible fungus. A mycorrhiza was established after infection through the rhizoids of the germinating seedling. Infection through the suspensor did occur, but these hyphae did not form pelotons, and embryo cells in contact with the hyphae were filled with a tannin‐like substance. Germination, and establishment of mycorrhiza, took about 14 days in vitro. From day 9 the protein reserves were hydrolysed, the protein vacuoles coalesced and starch accumulated in plastids. Certain epidermal cells developed nuclei about eight times original volume and produced rhizoids which emerged from day 11. After infection the hyphae formed pelotons in central cells with enlarged nuclei (16–64 times original volume). The intracellular hyphae developed close contacts with the hypertrophied host nuclei. Collapsed pelotons could be observed in cells from day 12, one day after infection. Meristematic activity in the uninfected chalazal end of the seedling began on day 12. On day 28 the first vascular tissue started to develop and on day 35 the beginning of a leafy shoot could be detected.
AB - Seeds of Dactylorhiza majalis (Rchb. f.) Hunt & Summerhayes were sown in vitro with a compatible fungus. A mycorrhiza was established after infection through the rhizoids of the germinating seedling. Infection through the suspensor did occur, but these hyphae did not form pelotons, and embryo cells in contact with the hyphae were filled with a tannin‐like substance. Germination, and establishment of mycorrhiza, took about 14 days in vitro. From day 9 the protein reserves were hydrolysed, the protein vacuoles coalesced and starch accumulated in plastids. Certain epidermal cells developed nuclei about eight times original volume and produced rhizoids which emerged from day 11. After infection the hyphae formed pelotons in central cells with enlarged nuclei (16–64 times original volume). The intracellular hyphae developed close contacts with the hypertrophied host nuclei. Collapsed pelotons could be observed in cells from day 12, one day after infection. Meristematic activity in the uninfected chalazal end of the seedling began on day 12. On day 28 the first vascular tissue started to develop and on day 35 the beginning of a leafy shoot could be detected.
KW - cell differentiation
KW - Dactylorhiza majalis
KW - Epulorhiza sp
KW - germination
KW - orchid mycorrhiza
UR - http://www.scopus.com/inward/record.url?scp=0025664722&partnerID=8YFLogxK
U2 - 10.1111/j.1469-8137.1990.tb00519.x
DO - 10.1111/j.1469-8137.1990.tb00519.x
M3 - Journal article
AN - SCOPUS:0025664722
VL - 116
SP - 137
EP - 147
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 1
ER -
ID: 209703110