Enzymatic solubilisation and degradation of soybean fibre demonstrated by viscosity, fibre analysis and microscopy

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Enzymatic solubilisation and degradation of soybean fibre demonstrated by viscosity, fibre analysis and microscopy. / Ravn, Jonas Laukkonen; Martens, Helle Juel; Pettersson, Dan; Pedersen, Ninfa.

In: Journal of Agricultural Science, Vol. 7, No. 9, 2015.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ravn, JL, Martens, HJ, Pettersson, D & Pedersen, N 2015, 'Enzymatic solubilisation and degradation of soybean fibre demonstrated by viscosity, fibre analysis and microscopy', Journal of Agricultural Science, vol. 7, no. 9. https://doi.org/10.5539/jas.v7n9p1

APA

Ravn, J. L., Martens, H. J., Pettersson, D., & Pedersen, N. (2015). Enzymatic solubilisation and degradation of soybean fibre demonstrated by viscosity, fibre analysis and microscopy. Journal of Agricultural Science, 7(9). https://doi.org/10.5539/jas.v7n9p1

Vancouver

Ravn JL, Martens HJ, Pettersson D, Pedersen N. Enzymatic solubilisation and degradation of soybean fibre demonstrated by viscosity, fibre analysis and microscopy. Journal of Agricultural Science. 2015;7(9). https://doi.org/10.5539/jas.v7n9p1

Author

Ravn, Jonas Laukkonen ; Martens, Helle Juel ; Pettersson, Dan ; Pedersen, Ninfa. / Enzymatic solubilisation and degradation of soybean fibre demonstrated by viscosity, fibre analysis and microscopy. In: Journal of Agricultural Science. 2015 ; Vol. 7, No. 9.

Bibtex

@article{5f108e35845a4af0ac51c76103a430a6,
title = "Enzymatic solubilisation and degradation of soybean fibre demonstrated by viscosity, fibre analysis and microscopy",
abstract = "The effect of a commercial multienzyme product obtained by fermentation from Aspergillus aculeatus on soybean and soybean meal was investigated using viscosity measurements, dietary fibre component analysis and different microscopy techniques utilizing histochemical dyes and antibody labelling. The results obtained demonstrated a strong viscosity reducing effect of the enzyme preparation on soluble galactomannan and xyloglucan polysaccharides and in addition non-starch polysaccharide analysis demonstrated a notable solubilisation of all polysaccharide constituents. The degradation of these components as native integral parts of cell walls upon exposure to the enzyme was visualized with microscopy. Two histochemical dyes, coriphosphine O and alcian blue were successfully used to follow pectin solubilisation after enzyme treatment. Commercial antibodies recognizing specific components of pectin and hemicellulose components of soybean cell wall were also used to visualize several enzyme activities in the commercial enzyme preparation The challenges of using commercial antibodies elicited from a given plant source to detect similar epitiopes on another plant source are also discussed. Non-starch polysaccharide analysis of the insoluble dietary fibre constituents before and after enzyme treatment corroborated the visualized mode of action demonstrated by microscopy. The combination of techniques provided visual and quantitative measurements of the solubilisation and degradation of hemicellulose pectic soybean cell wall components as part of the undesirable antinutrients in animal feed.",
author = "Ravn, {Jonas Laukkonen} and Martens, {Helle Juel} and Dan Pettersson and Ninfa Pedersen",
year = "2015",
doi = "10.5539/jas.v7n9p1",
language = "English",
volume = "7",
journal = "Journal of Agricultural Science",
issn = "1916-9752",
publisher = "Canadian Center of Science and Education (CCSE)",
number = "9",

}

RIS

TY - JOUR

T1 - Enzymatic solubilisation and degradation of soybean fibre demonstrated by viscosity, fibre analysis and microscopy

AU - Ravn, Jonas Laukkonen

AU - Martens, Helle Juel

AU - Pettersson, Dan

AU - Pedersen, Ninfa

PY - 2015

Y1 - 2015

N2 - The effect of a commercial multienzyme product obtained by fermentation from Aspergillus aculeatus on soybean and soybean meal was investigated using viscosity measurements, dietary fibre component analysis and different microscopy techniques utilizing histochemical dyes and antibody labelling. The results obtained demonstrated a strong viscosity reducing effect of the enzyme preparation on soluble galactomannan and xyloglucan polysaccharides and in addition non-starch polysaccharide analysis demonstrated a notable solubilisation of all polysaccharide constituents. The degradation of these components as native integral parts of cell walls upon exposure to the enzyme was visualized with microscopy. Two histochemical dyes, coriphosphine O and alcian blue were successfully used to follow pectin solubilisation after enzyme treatment. Commercial antibodies recognizing specific components of pectin and hemicellulose components of soybean cell wall were also used to visualize several enzyme activities in the commercial enzyme preparation The challenges of using commercial antibodies elicited from a given plant source to detect similar epitiopes on another plant source are also discussed. Non-starch polysaccharide analysis of the insoluble dietary fibre constituents before and after enzyme treatment corroborated the visualized mode of action demonstrated by microscopy. The combination of techniques provided visual and quantitative measurements of the solubilisation and degradation of hemicellulose pectic soybean cell wall components as part of the undesirable antinutrients in animal feed.

AB - The effect of a commercial multienzyme product obtained by fermentation from Aspergillus aculeatus on soybean and soybean meal was investigated using viscosity measurements, dietary fibre component analysis and different microscopy techniques utilizing histochemical dyes and antibody labelling. The results obtained demonstrated a strong viscosity reducing effect of the enzyme preparation on soluble galactomannan and xyloglucan polysaccharides and in addition non-starch polysaccharide analysis demonstrated a notable solubilisation of all polysaccharide constituents. The degradation of these components as native integral parts of cell walls upon exposure to the enzyme was visualized with microscopy. Two histochemical dyes, coriphosphine O and alcian blue were successfully used to follow pectin solubilisation after enzyme treatment. Commercial antibodies recognizing specific components of pectin and hemicellulose components of soybean cell wall were also used to visualize several enzyme activities in the commercial enzyme preparation The challenges of using commercial antibodies elicited from a given plant source to detect similar epitiopes on another plant source are also discussed. Non-starch polysaccharide analysis of the insoluble dietary fibre constituents before and after enzyme treatment corroborated the visualized mode of action demonstrated by microscopy. The combination of techniques provided visual and quantitative measurements of the solubilisation and degradation of hemicellulose pectic soybean cell wall components as part of the undesirable antinutrients in animal feed.

U2 - 10.5539/jas.v7n9p1

DO - 10.5539/jas.v7n9p1

M3 - Journal article

VL - 7

JO - Journal of Agricultural Science

JF - Journal of Agricultural Science

SN - 1916-9752

IS - 9

ER -

ID: 146776714