Insights into carbon metabolism provided by fluorescence in situ hybridization-secondary ion mass spectrometry imaging of an autotrophic, nitrate-reducing, Fe(II)-oxidizing enrichment culture

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Standard

Insights into carbon metabolism provided by fluorescence in situ hybridization-secondary ion mass spectrometry imaging of an autotrophic, nitrate-reducing, Fe(II)-oxidizing enrichment culture. / Tominski, Claudia; Lösekann-Behrens, Tina; Ruecker, Alexander; Hagemann, Nikolas; Kleindienst, Sara; Mueller, Carsten W.; Höschen, Carmen; Kögel-Knabner, Ingrid; Kappler, Andreas; Behrens, Sebastian.

In: Applied and Environmental Microbiology, Vol. 84, No. 9, e02166-17, 2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Tominski, C, Lösekann-Behrens, T, Ruecker, A, Hagemann, N, Kleindienst, S, Mueller, CW, Höschen, C, Kögel-Knabner, I, Kappler, A & Behrens, S 2018, 'Insights into carbon metabolism provided by fluorescence in situ hybridization-secondary ion mass spectrometry imaging of an autotrophic, nitrate-reducing, Fe(II)-oxidizing enrichment culture', Applied and Environmental Microbiology, vol. 84, no. 9, e02166-17. https://doi.org/10.1128/AEM.02166-17

APA

Tominski, C., Lösekann-Behrens, T., Ruecker, A., Hagemann, N., Kleindienst, S., Mueller, C. W., Höschen, C., Kögel-Knabner, I., Kappler, A., & Behrens, S. (2018). Insights into carbon metabolism provided by fluorescence in situ hybridization-secondary ion mass spectrometry imaging of an autotrophic, nitrate-reducing, Fe(II)-oxidizing enrichment culture. Applied and Environmental Microbiology, 84(9), [e02166-17]. https://doi.org/10.1128/AEM.02166-17

Vancouver

Tominski C, Lösekann-Behrens T, Ruecker A, Hagemann N, Kleindienst S, Mueller CW et al. Insights into carbon metabolism provided by fluorescence in situ hybridization-secondary ion mass spectrometry imaging of an autotrophic, nitrate-reducing, Fe(II)-oxidizing enrichment culture. Applied and Environmental Microbiology. 2018;84(9). e02166-17. https://doi.org/10.1128/AEM.02166-17

Author

Tominski, Claudia ; Lösekann-Behrens, Tina ; Ruecker, Alexander ; Hagemann, Nikolas ; Kleindienst, Sara ; Mueller, Carsten W. ; Höschen, Carmen ; Kögel-Knabner, Ingrid ; Kappler, Andreas ; Behrens, Sebastian. / Insights into carbon metabolism provided by fluorescence in situ hybridization-secondary ion mass spectrometry imaging of an autotrophic, nitrate-reducing, Fe(II)-oxidizing enrichment culture. In: Applied and Environmental Microbiology. 2018 ; Vol. 84, No. 9.

Bibtex

@article{af86fc3b6a0e48d98ae790bb49ae2a9e,
title = "Insights into carbon metabolism provided by fluorescence in situ hybridization-secondary ion mass spectrometry imaging of an autotrophic, nitrate-reducing, Fe(II)-oxidizing enrichment culture",
abstract = "The enrichment culture KS is one of the few existing autotrophic, nitrate-reducing, Fe(II)-oxidizing cultures that can be continuously transferred without an organic carbon source. We used a combination of catalyzed amplification reporter deposition fluorescence in situ hybridization (CARD-FISH) and nanoscale secondary ion mass spectrometry (NanoSIMS) to analyze community dynamics, singlecell activities, and interactions among the two most abundant microbial community members (i.e., Gallionellaceae sp. and Bradyrhizobium spp.) under autotrophic and heterotrophic growth conditions. CARD-FISH cell counts showed the dominance of the Fe(II) oxidizer Gallionellaceae sp. under autotrophic conditions as well as of Bradyrhizobium spp. under heterotrophic conditions. We used NanoSIMS to monitor the fate of 13C-labeled bicarbonate and acetate as well as 15N-labeled ammonium at the single-cell level for both taxa. Under autotrophic conditions, only the Gallionellaceae sp. was actively incorporating 13C-labeled bicarbonate and 15N-labeled ammonium. Interestingly, both Bradyrhizobium spp. and Gallionellaceae sp. became enriched in [13C]acetate and [15N]ammonium under heterotrophic conditions. Our experiments demonstrated that Gallionellaceae sp. was capable of assimilating [13C]acetate while Bradyrhizobium spp. were not able to fix CO2, although a metagenomics survey of culture KS recently revealed that Gallionellaceae sp. lacks genes for acetate uptake and that the Bradyrhizobium sp. carries the genetic potential to fix CO2. The study furthermore extends our understanding of the microbial reactions that interlink the nitrogen and Fe cycles in the environment.",
keywords = "Bradyrhizobium, Chemolithoautotrophic ferrous iron [Fe(II)] oxidation, Fluorescence in situ hybridization (FISH), Gallionellaceae, Nanoscale secondary-ion mass spectrometry (NanoSIMS), Neutrophilic, Nitrate-dependent Fe(II) oxidation (NDFO)",
author = "Claudia Tominski and Tina L{\"o}sekann-Behrens and Alexander Ruecker and Nikolas Hagemann and Sara Kleindienst and Mueller, {Carsten W.} and Carmen H{\"o}schen and Ingrid K{\"o}gel-Knabner and Andreas Kappler and Sebastian Behrens",
year = "2018",
doi = "10.1128/AEM.02166-17",
language = "English",
volume = "84",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "9",

}

RIS

TY - JOUR

T1 - Insights into carbon metabolism provided by fluorescence in situ hybridization-secondary ion mass spectrometry imaging of an autotrophic, nitrate-reducing, Fe(II)-oxidizing enrichment culture

AU - Tominski, Claudia

AU - Lösekann-Behrens, Tina

AU - Ruecker, Alexander

AU - Hagemann, Nikolas

AU - Kleindienst, Sara

AU - Mueller, Carsten W.

AU - Höschen, Carmen

AU - Kögel-Knabner, Ingrid

AU - Kappler, Andreas

AU - Behrens, Sebastian

PY - 2018

Y1 - 2018

N2 - The enrichment culture KS is one of the few existing autotrophic, nitrate-reducing, Fe(II)-oxidizing cultures that can be continuously transferred without an organic carbon source. We used a combination of catalyzed amplification reporter deposition fluorescence in situ hybridization (CARD-FISH) and nanoscale secondary ion mass spectrometry (NanoSIMS) to analyze community dynamics, singlecell activities, and interactions among the two most abundant microbial community members (i.e., Gallionellaceae sp. and Bradyrhizobium spp.) under autotrophic and heterotrophic growth conditions. CARD-FISH cell counts showed the dominance of the Fe(II) oxidizer Gallionellaceae sp. under autotrophic conditions as well as of Bradyrhizobium spp. under heterotrophic conditions. We used NanoSIMS to monitor the fate of 13C-labeled bicarbonate and acetate as well as 15N-labeled ammonium at the single-cell level for both taxa. Under autotrophic conditions, only the Gallionellaceae sp. was actively incorporating 13C-labeled bicarbonate and 15N-labeled ammonium. Interestingly, both Bradyrhizobium spp. and Gallionellaceae sp. became enriched in [13C]acetate and [15N]ammonium under heterotrophic conditions. Our experiments demonstrated that Gallionellaceae sp. was capable of assimilating [13C]acetate while Bradyrhizobium spp. were not able to fix CO2, although a metagenomics survey of culture KS recently revealed that Gallionellaceae sp. lacks genes for acetate uptake and that the Bradyrhizobium sp. carries the genetic potential to fix CO2. The study furthermore extends our understanding of the microbial reactions that interlink the nitrogen and Fe cycles in the environment.

AB - The enrichment culture KS is one of the few existing autotrophic, nitrate-reducing, Fe(II)-oxidizing cultures that can be continuously transferred without an organic carbon source. We used a combination of catalyzed amplification reporter deposition fluorescence in situ hybridization (CARD-FISH) and nanoscale secondary ion mass spectrometry (NanoSIMS) to analyze community dynamics, singlecell activities, and interactions among the two most abundant microbial community members (i.e., Gallionellaceae sp. and Bradyrhizobium spp.) under autotrophic and heterotrophic growth conditions. CARD-FISH cell counts showed the dominance of the Fe(II) oxidizer Gallionellaceae sp. under autotrophic conditions as well as of Bradyrhizobium spp. under heterotrophic conditions. We used NanoSIMS to monitor the fate of 13C-labeled bicarbonate and acetate as well as 15N-labeled ammonium at the single-cell level for both taxa. Under autotrophic conditions, only the Gallionellaceae sp. was actively incorporating 13C-labeled bicarbonate and 15N-labeled ammonium. Interestingly, both Bradyrhizobium spp. and Gallionellaceae sp. became enriched in [13C]acetate and [15N]ammonium under heterotrophic conditions. Our experiments demonstrated that Gallionellaceae sp. was capable of assimilating [13C]acetate while Bradyrhizobium spp. were not able to fix CO2, although a metagenomics survey of culture KS recently revealed that Gallionellaceae sp. lacks genes for acetate uptake and that the Bradyrhizobium sp. carries the genetic potential to fix CO2. The study furthermore extends our understanding of the microbial reactions that interlink the nitrogen and Fe cycles in the environment.

KW - Bradyrhizobium

KW - Chemolithoautotrophic ferrous iron [Fe(II)] oxidation

KW - Fluorescence in situ hybridization (FISH)

KW - Gallionellaceae

KW - Nanoscale secondary-ion mass spectrometry (NanoSIMS)

KW - Neutrophilic

KW - Nitrate-dependent Fe(II) oxidation (NDFO)

U2 - 10.1128/AEM.02166-17

DO - 10.1128/AEM.02166-17

M3 - Journal article

C2 - 29500258

AN - SCOPUS:85045506772

VL - 84

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 9

M1 - e02166-17

ER -

ID: 238951830