Co-occurring orchid species associated with different low-abundance mycorrhizal fungi from the soil in a high-diversity conservation area in Denmark
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Co-occurring orchid species associated with different low-abundance mycorrhizal fungi from the soil in a high-diversity conservation area in Denmark. / Hartvig, Ida; Kosawang, Chatchai; Rasmussen, Hanne; Kjær, Erik Dahl; Nielsen, Lene Rostgaard.
I: Ecology and Evolution, Bind 14, Nr. 2, e10863, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Co-occurring orchid species associated with different low-abundance mycorrhizal fungi from the soil in a high-diversity conservation area in Denmark
AU - Hartvig, Ida
AU - Kosawang, Chatchai
AU - Rasmussen, Hanne
AU - Kjær, Erik Dahl
AU - Nielsen, Lene Rostgaard
N1 - Funding Information: We thank Lene Hasmark Andersen for extracting DNA from soil samples. Melissa McCormick is thanked for providing information on suitable OMF primers and PCR conditions. Lea Vig McKinney helped screening seed packets for protocorms. We are grateful to Danish Nature Agency in Storstrøm for collaboration and access to field sites throughout the project. The project was supported by Godfred Birkedal Hartmanns Familiefond and infrastructure at the Center for Evolutionary Hologenomics was funded by the Danish National Research Foundation grant DNRF143. Funding Information: We thank Lene Hasmark Andersen for extracting DNA from soil samples. Melissa McCormick is thanked for providing information on suitable OMF primers and PCR conditions. Lea Vig McKinney helped screening seed packets for protocorms. We are grateful to Danish Nature Agency in Storstrøm for collaboration and access to field sites throughout the project. The project was supported by Godfred Birkedal Hartmanns Familiefond and infrastructure at the Center for Evolutionary Hologenomics was funded by the Danish National Research Foundation grant DNRF143. Publisher Copyright: © 2024 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.
PY - 2024
Y1 - 2024
N2 - Plant–fungal interactions are ubiquitous across ecosystems and contribute significantly to plant ecology and evolution. All orchids form obligate symbiotic relationships with specific fungi for germination and early growth, and the distribution of terrestrial orchid species has been linked to occurrence and abundance of specific orchid mycorrhizal fungi (OMF) in the soil. The availability of OMF can therefore be a habitat requirement that is relevant to consider when establishing management and conservation strategies for threatened orchid species, but knowledge on the spatial distribution of OMF in soil is limited. We here studied the mycorrhizal associations of three terrestrial orchid species (Anacamptis pyramidalis, Orchis purpurea and Platanthera chlorantha) found in a local orchid diversity hotspot in eastern Denmark, and investigated the abundance of the identified mycorrhizal fungi in the surrounding soil. We applied ITS metabarcoding to samples of orchid roots, rhizosphere soil and bulk soil collected at three localities, supplemented with standard barcoding of root samples with OMF specific primers, and detected 22 Operational Taxonomic Units (OTUs) putatively identified as OMF. The three orchid species displayed different patterns of OMF associations, supporting the theory that association with specific fungi constitutes part of an orchid's ecological niche allowing co-occurrence of many species in orchid-rich habitats. The identified mycorrhizal partners in the basidiomycete families Tulasnellaceae and Ceratobasidiaceae (Cantharallales) were detected in low abundance in rhizosphere soil, and appeared almost absent from bulk soil at the localities. This finding highlights our limited knowledge of the ecology and trophic mode of OMF outside orchid tissues, as well as challenges in the detection of specific OMF with standard methods. Potential implications for management and conservation strategies are discussed.
AB - Plant–fungal interactions are ubiquitous across ecosystems and contribute significantly to plant ecology and evolution. All orchids form obligate symbiotic relationships with specific fungi for germination and early growth, and the distribution of terrestrial orchid species has been linked to occurrence and abundance of specific orchid mycorrhizal fungi (OMF) in the soil. The availability of OMF can therefore be a habitat requirement that is relevant to consider when establishing management and conservation strategies for threatened orchid species, but knowledge on the spatial distribution of OMF in soil is limited. We here studied the mycorrhizal associations of three terrestrial orchid species (Anacamptis pyramidalis, Orchis purpurea and Platanthera chlorantha) found in a local orchid diversity hotspot in eastern Denmark, and investigated the abundance of the identified mycorrhizal fungi in the surrounding soil. We applied ITS metabarcoding to samples of orchid roots, rhizosphere soil and bulk soil collected at three localities, supplemented with standard barcoding of root samples with OMF specific primers, and detected 22 Operational Taxonomic Units (OTUs) putatively identified as OMF. The three orchid species displayed different patterns of OMF associations, supporting the theory that association with specific fungi constitutes part of an orchid's ecological niche allowing co-occurrence of many species in orchid-rich habitats. The identified mycorrhizal partners in the basidiomycete families Tulasnellaceae and Ceratobasidiaceae (Cantharallales) were detected in low abundance in rhizosphere soil, and appeared almost absent from bulk soil at the localities. This finding highlights our limited knowledge of the ecology and trophic mode of OMF outside orchid tissues, as well as challenges in the detection of specific OMF with standard methods. Potential implications for management and conservation strategies are discussed.
KW - Ceratobasidiaceae
KW - ITS metabarcoding
KW - orchid mycorrhizal fungi
KW - plant hologenomics
KW - soil mycobiome
KW - Tulasnellaceae
U2 - 10.1002/ece3.10863
DO - 10.1002/ece3.10863
M3 - Journal article
C2 - 38304271
AN - SCOPUS:85183932446
VL - 14
JO - Ecology and Evolution
JF - Ecology and Evolution
SN - 2045-7758
IS - 2
M1 - e10863
ER -
ID: 385021872