Methods for visualising active microbial benzene degraders in in situ microcosms
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Methods for visualising active microbial benzene degraders in in situ microcosms. / Schurig, Christian; Mueller, Carsten W.; Höschen, Carmen; Prager, Andrea; Kothe, Erika; Beck, Henrike; Miltner, Anja; Kästner, Matthias.
I: Applied Microbiology and Biotechnology, Bind 99, Nr. 2, 2015, s. 957-968.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Methods for visualising active microbial benzene degraders in in situ microcosms
AU - Schurig, Christian
AU - Mueller, Carsten W.
AU - Höschen, Carmen
AU - Prager, Andrea
AU - Kothe, Erika
AU - Beck, Henrike
AU - Miltner, Anja
AU - Kästner, Matthias
PY - 2015
Y1 - 2015
N2 - Natural attenuation maybe a cost-efficient option for bioremediation of contaminated sites but requires knowledge about the activity of degrading microbes under in situ conditions. In order to link microbial activity to the spatial distribution of contaminant degraders, we combined the recently improved in situ microcosm approach, so-called ‘direct-push bacterial trap’ (DP-BACTRAP), with nano-scale secondary ion mass spectrometry (NanoSIMS) analysis on samples from contaminated constructed wetlands. This approach is based on initially sterile microcosms amended with 13C-labelled benzene as a source of carbon and energy for microorganisms. The microcosms were introduced directly in the constructed wetland, where they were colonised by indigenous microorganisms from the sediment. After incubation in the field, the samples were analysed by NanoSIMS, scanning electron microscopy (SEM) and fluorescence microscopy in order to visualise 13C-labelled microbial biomass on undisturbed samples from the microcosms. With the approach developed, we successfully visualised benzene-degrading microbes on solid materials with high surface area by means of NanoSIMS. Moreover, we could demonstrate the feasibility of NanoSIMS analysis of unembedded porous media with a highly complex topography, which was frequently reasoned to not lead to sufficient results.
AB - Natural attenuation maybe a cost-efficient option for bioremediation of contaminated sites but requires knowledge about the activity of degrading microbes under in situ conditions. In order to link microbial activity to the spatial distribution of contaminant degraders, we combined the recently improved in situ microcosm approach, so-called ‘direct-push bacterial trap’ (DP-BACTRAP), with nano-scale secondary ion mass spectrometry (NanoSIMS) analysis on samples from contaminated constructed wetlands. This approach is based on initially sterile microcosms amended with 13C-labelled benzene as a source of carbon and energy for microorganisms. The microcosms were introduced directly in the constructed wetland, where they were colonised by indigenous microorganisms from the sediment. After incubation in the field, the samples were analysed by NanoSIMS, scanning electron microscopy (SEM) and fluorescence microscopy in order to visualise 13C-labelled microbial biomass on undisturbed samples from the microcosms. With the approach developed, we successfully visualised benzene-degrading microbes on solid materials with high surface area by means of NanoSIMS. Moreover, we could demonstrate the feasibility of NanoSIMS analysis of unembedded porous media with a highly complex topography, which was frequently reasoned to not lead to sufficient results.
KW - C
KW - Benzene
KW - DP-BACTRAPs
KW - In situ microcosms
KW - Microbial activity
KW - NanoSIMS
U2 - 10.1007/s00253-014-6037-4
DO - 10.1007/s00253-014-6037-4
M3 - Journal article
C2 - 25194840
AN - SCOPUS:84921751716
VL - 99
SP - 957
EP - 968
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 2
ER -
ID: 239161480