NeuroD1: developmental expression and regulated genes in the rodent pineal gland
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
NeuroD1: developmental expression and regulated genes in the rodent pineal gland. / Muñoz, Estela M; Bailey, Michael J; Rath, Martin F; Shi, Qiong; Morin, Fabrice; Coon, Steven L; Møller, Morten; Klein, David C.
I: Journal of Neurochemistry, Bind 102, Nr. 3, 2007, s. 887-899.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - NeuroD1: developmental expression and regulated genes in the rodent pineal gland
AU - Muñoz, Estela M
AU - Bailey, Michael J
AU - Rath, Martin F
AU - Shi, Qiong
AU - Morin, Fabrice
AU - Coon, Steven L
AU - Møller, Morten
AU - Klein, David C
N1 - Paper id:: PMID: 17630985
PY - 2007
Y1 - 2007
N2 - NeuroD1/BETA2, a member of the bHLH transcription factor family, is known to influence the fate of specific neuronal, endocrine and retinal cells. We report here that NeuroD1 mRNA is highly abundant in the developing and adult rat pineal gland. Pineal expression begins in the 17-day embryo at which time it is also detectable in other brain regions. Expression in the pineal gland increases during the embryonic period and is maintained thereafter at levels equivalent to those found in the cerebellum and retina. In contrast, NeuroD1 mRNA decreases markedly in non-cerebellar brain regions during development. Pineal NeuroD1 levels are similar during the day and night, and do not appear to be influenced by sympathetic neural input. Gene expression analysis of the pineal glands from neonatal NeuroD1 knockout mice identifies 127 transcripts that are down-regulated (>twofold, p <0.05) and 16 that are up-regulated (>twofold, p <0.05). According to quantitative RT-PCR, the most dramatically down-regulated gene is kinesin family member 5C ( approximately 100-fold) and the most dramatically up-regulated gene is glutamic acid decarboxylase 1 ( approximately fourfold). Other impacted transcripts encode proteins involved in differentiation, development, signal transduction and trafficking. These findings represent the first step toward elucidating the role of NeuroD1 in the rodent pinealocyte.
AB - NeuroD1/BETA2, a member of the bHLH transcription factor family, is known to influence the fate of specific neuronal, endocrine and retinal cells. We report here that NeuroD1 mRNA is highly abundant in the developing and adult rat pineal gland. Pineal expression begins in the 17-day embryo at which time it is also detectable in other brain regions. Expression in the pineal gland increases during the embryonic period and is maintained thereafter at levels equivalent to those found in the cerebellum and retina. In contrast, NeuroD1 mRNA decreases markedly in non-cerebellar brain regions during development. Pineal NeuroD1 levels are similar during the day and night, and do not appear to be influenced by sympathetic neural input. Gene expression analysis of the pineal glands from neonatal NeuroD1 knockout mice identifies 127 transcripts that are down-regulated (>twofold, p <0.05) and 16 that are up-regulated (>twofold, p <0.05). According to quantitative RT-PCR, the most dramatically down-regulated gene is kinesin family member 5C ( approximately 100-fold) and the most dramatically up-regulated gene is glutamic acid decarboxylase 1 ( approximately fourfold). Other impacted transcripts encode proteins involved in differentiation, development, signal transduction and trafficking. These findings represent the first step toward elucidating the role of NeuroD1 in the rodent pinealocyte.
KW - Animals
KW - Basic Helix-Loop-Helix Transcription Factors
KW - Brain
KW - Circadian Rhythm
KW - Down-Regulation
KW - Gene Expression Regulation, Developmental
KW - Glutamate Decarboxylase
KW - Kinesin
KW - Male
KW - Mice
KW - Mice, Knockout
KW - Pineal Gland
KW - RNA, Messenger
KW - Rats
KW - Rats, Sprague-Dawley
U2 - 10.1111/j.1471-4159.2007.04605.x
DO - 10.1111/j.1471-4159.2007.04605.x
M3 - Journal article
C2 - 17630985
VL - 102
SP - 887
EP - 899
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 3
ER -
ID: 18316090