Accumulated effects of factors determining plant development from somatic embryos of Abies nordmanniana and Abies bornmuelleriana
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Accumulated effects of factors determining plant development from somatic embryos of Abies nordmanniana and Abies bornmuelleriana. / Nielsen, Ulrik Braüner; Hansen, Camilla Bülow; Hansen, Ulrich; Johansen, Vivian Kvist; Egertsdotter, Ulrika.
In: Frontiers in Plant Science, Vol. 13, 989484, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Accumulated effects of factors determining plant development from somatic embryos of Abies nordmanniana and Abies bornmuelleriana
AU - Nielsen, Ulrik Braüner
AU - Hansen, Camilla Bülow
AU - Hansen, Ulrich
AU - Johansen, Vivian Kvist
AU - Egertsdotter, Ulrika
N1 - Publisher Copyright: Copyright © 2022 Nielsen, Hansen, Hansen, Johansen and Egertsdotter.
PY - 2022
Y1 - 2022
N2 - Despite a much later inception of somatic embryogenesis (SE) propagation protocols for gymnosperms than for angiosperm species, SE is becoming increasingly important due to its applications for commercial forestry. For many conifers, there are however still major bottlenecks in the SE plant production process limiting the use of SE for forestry operations, Christmas tree production and research projects. In the present case study, the effects on plant growth from different cultural factors applied during the SE developmental process were studied in two conifer species of high value for Christmas tree production. Seven clones of Abies nordmanniana and two clones of Abies bornmuelleriana were included in the study. Accumulated effects from cultural treatments were recorded from the start of germination of mature embryos of different quality scores through development into plants in the third growing period. Experimental factors of the cultural treatments included were: germination temperature, germination time, light conditions, survival ex vitro and traits for plant growth and vitality. The results reveal that most of the studied experimental factors influenced plant growth during the first three years however their relative importance was different. Plant survival rate at end of the nursery stage was strongly impacted by germination temperature (p<0.001), initial embryo score (p=0.007), clone (p<0.001) and to a lesser extend week of germination (p=0.017). This case-study highlights and quantifies the strong interrelation between the developmental steps of somatic embryogenesis and show the importance of considering all cultural steps when optimizing SE plant production protocols.
AB - Despite a much later inception of somatic embryogenesis (SE) propagation protocols for gymnosperms than for angiosperm species, SE is becoming increasingly important due to its applications for commercial forestry. For many conifers, there are however still major bottlenecks in the SE plant production process limiting the use of SE for forestry operations, Christmas tree production and research projects. In the present case study, the effects on plant growth from different cultural factors applied during the SE developmental process were studied in two conifer species of high value for Christmas tree production. Seven clones of Abies nordmanniana and two clones of Abies bornmuelleriana were included in the study. Accumulated effects from cultural treatments were recorded from the start of germination of mature embryos of different quality scores through development into plants in the third growing period. Experimental factors of the cultural treatments included were: germination temperature, germination time, light conditions, survival ex vitro and traits for plant growth and vitality. The results reveal that most of the studied experimental factors influenced plant growth during the first three years however their relative importance was different. Plant survival rate at end of the nursery stage was strongly impacted by germination temperature (p<0.001), initial embryo score (p=0.007), clone (p<0.001) and to a lesser extend week of germination (p=0.017). This case-study highlights and quantifies the strong interrelation between the developmental steps of somatic embryogenesis and show the importance of considering all cultural steps when optimizing SE plant production protocols.
KW - abies
KW - conifer
KW - embryo quality
KW - germination
KW - in vitro
KW - nordmann fir
KW - turkish fir
U2 - 10.3389/fpls.2022.989484
DO - 10.3389/fpls.2022.989484
M3 - Journal article
C2 - 36311146
AN - SCOPUS:85140760945
VL - 13
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 989484
ER -
ID: 325022921