Impact of lignins isolated from pretreated lignocelluloses on enzymatic cellulose saccharification

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Søren Talbro Barsberg
  • Michael Joseph Selig
  • Claus Felby
Lignins were enzymatically isolated from corn stover and wheat straw samples and subjected to hydrothermal or wet oxidation pretreatments for enzyme adsorption experimentations. Lignin contents of the isolates ranged from 26 to 71 % (w/w); cellulose ranged from 3 to 22 % (w/w); xylan from 0.7 to 6 % (w/w) and ash was from 5.8 to 30 % (w/w). ATR-IR analyses indicated significant and similar levels of calcium in all lignin isolates. Commercial cellulase adsorption studies showed that the presence of these lignins had no significant impact on the total amount of adsorbed enzyme in cellulose and cellulose-lignin systems. Consequently, the presence of the lignins had minimal effect, if any, on enzymatic cellulose conversion. Furthermore, this result, coupled with significant calcium levels in the isolated lignins, supports previous work suggesting lignin-calcium complexes reduce enzyme-lignin interactions.
OriginalsprogEngelsk
TidsskriftBiotechnology Letters
Vol/bind35
Udgave nummer2
Sider (fra-til)189-195
Antal sider7
ISSN0141-5492
DOI
StatusUdgivet - 2013

ID: 97187638