4D Surface Reconstructions to Study Microscale Structures and Functions in Soil Biogeochemistry
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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4D Surface Reconstructions to Study Microscale Structures and Functions in Soil Biogeochemistry. / Ost, Alexander D; Wu, Tianyi; Höschen, Carmen; Mueller, Carsten W; Wirtz, Tom; Audinot, Jean-Nicolas.
I: Environmental Science & Technology, Bind 55, Nr. 13, 06.07.2021, s. 9384-9393.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - 4D Surface Reconstructions to Study Microscale Structures and Functions in Soil Biogeochemistry
AU - Ost, Alexander D
AU - Wu, Tianyi
AU - Höschen, Carmen
AU - Mueller, Carsten W
AU - Wirtz, Tom
AU - Audinot, Jean-Nicolas
PY - 2021/7/6
Y1 - 2021/7/6
N2 - The development of high-resolution microscopy and spectroscopy techniques has allowed the analysis of microscopic 3D objects in fields like nanotechnology and life and soil sciences. Soils have the ability to incorporate and store large amounts of organic carbon. To study this organic matter (OM) sequestration, it is essential to analyze its association with soil minerals at the relevant microaggregate scale. This has been previously studied in 2D. However, 3D surface representations would allow a variable angle and magnification analysis, providing detailed insight on their architecture. Here we illustrate a 4D surface reconstruction workflow able to locate preferential sites for OM deposition with respect to microaggregate topography. We used Helium Ion Microscopy to acquire overlapping Secondary Electron (SE) images to reconstruct the soil topography in 3D. Then we used nanoscale Secondary Ion Mass Spectrometry imaging to chemically differentiate between the OM and mineral constituents forming the microaggregates. This image was projected onto the 3D SE model to create a 4D surface reconstruction. Our results show that organo-mineral associations mainly form at medium curvatures while flat and highly curved surfaces are avoided. This method presents an important step forward to survey the 3D physical structure and chemical composition of microscale biogeochemical systems correlatively.
AB - The development of high-resolution microscopy and spectroscopy techniques has allowed the analysis of microscopic 3D objects in fields like nanotechnology and life and soil sciences. Soils have the ability to incorporate and store large amounts of organic carbon. To study this organic matter (OM) sequestration, it is essential to analyze its association with soil minerals at the relevant microaggregate scale. This has been previously studied in 2D. However, 3D surface representations would allow a variable angle and magnification analysis, providing detailed insight on their architecture. Here we illustrate a 4D surface reconstruction workflow able to locate preferential sites for OM deposition with respect to microaggregate topography. We used Helium Ion Microscopy to acquire overlapping Secondary Electron (SE) images to reconstruct the soil topography in 3D. Then we used nanoscale Secondary Ion Mass Spectrometry imaging to chemically differentiate between the OM and mineral constituents forming the microaggregates. This image was projected onto the 3D SE model to create a 4D surface reconstruction. Our results show that organo-mineral associations mainly form at medium curvatures while flat and highly curved surfaces are avoided. This method presents an important step forward to survey the 3D physical structure and chemical composition of microscale biogeochemical systems correlatively.
KW - Carbon
KW - Minerals
KW - Soil
KW - Spectrum Analysis
KW - NanoSIMS
KW - Soil organic carbon
KW - mineral-associated organic matter
KW - microaggregates
KW - soil carbon cycling
U2 - 10.1021/acs.est.1c02971
DO - 10.1021/acs.est.1c02971
M3 - Journal article
C2 - 34165287
VL - 55
SP - 9384
EP - 9393
JO - Environmental Science & Technology
JF - Environmental Science & Technology
SN - 0013-936X
IS - 13
ER -
ID: 274429678