Immunohistochemistry of the intercellular matrix components and the epithelio-mesenchymal junction of the human tooth germ
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Immunohistochemistry of the intercellular matrix components and the epithelio-mesenchymal junction of the human tooth germ. / Matthiessen, M E; Olsen, B E; Moe, D; Kirkeby, S.
I: Journal of Molecular Histology (Print Edition), Bind 26, Nr. 2, 1994, s. 110-8.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Immunohistochemistry of the intercellular matrix components and the epithelio-mesenchymal junction of the human tooth germ
AU - Matthiessen, M E
AU - Olsen, B E
AU - Moe, D
AU - Kirkeby, S
N1 - Keywords: Ameloblasts; Cell Adhesion Molecules, Neuronal; Collagen; Dental Papilla; Dentin; Extracellular Matrix Proteins; Fetus; Fibronectins; Heparitin Sulfate; Humans; Immunohistochemistry; Laminin; Odontogenesis; Tenascin; Tooth Germ
PY - 1994
Y1 - 1994
N2 - The immunohistochemical localization of heparan sulphate, collagen type I, III and IV, laminin, tenascin, plasma- and cellular fibronectin was studied in tooth germs from human fetuses. The lamina basalis ameloblastica or membrana preformativa, which separates the pre-ameloblasts from the pre-dentin and dentin, contained heparan sulphate, collagen type IV, laminin and fibronectin. Enamel reacted with antifibronectin, but the reaction varied depending on the type of fibronectin and the source of antibody. In early pre-dentin, collagen type I, laminin, tenascin and fibronectin were present. In late pre-dentin and dentin collagen type I was found in intertubular dentin and in the zone between enamel and dentin. The close relationship between collagen type I in dentin and fibronectin in immature enamel is interesting, as it may contribute to the stabilization of the amelodentinal interface. In dental pulp, collagen type IV and laminin were found in the endothelial basement membranes. Collagen type I and III, tenascin and fibronectin were localized to the mesenchymal intercellular matrix. The results of this study have supported the assumption that the lamina basalis ameloblastica is a basement membrane, and have lead to the suggestion that ameloblasts are producers of fibronectin or a fibronectin-like substance.
AB - The immunohistochemical localization of heparan sulphate, collagen type I, III and IV, laminin, tenascin, plasma- and cellular fibronectin was studied in tooth germs from human fetuses. The lamina basalis ameloblastica or membrana preformativa, which separates the pre-ameloblasts from the pre-dentin and dentin, contained heparan sulphate, collagen type IV, laminin and fibronectin. Enamel reacted with antifibronectin, but the reaction varied depending on the type of fibronectin and the source of antibody. In early pre-dentin, collagen type I, laminin, tenascin and fibronectin were present. In late pre-dentin and dentin collagen type I was found in intertubular dentin and in the zone between enamel and dentin. The close relationship between collagen type I in dentin and fibronectin in immature enamel is interesting, as it may contribute to the stabilization of the amelodentinal interface. In dental pulp, collagen type IV and laminin were found in the endothelial basement membranes. Collagen type I and III, tenascin and fibronectin were localized to the mesenchymal intercellular matrix. The results of this study have supported the assumption that the lamina basalis ameloblastica is a basement membrane, and have lead to the suggestion that ameloblasts are producers of fibronectin or a fibronectin-like substance.
M3 - Journal article
C2 - 7512084
VL - 26
SP - 110
EP - 118
JO - Journal of Molecular Histology
JF - Journal of Molecular Histology
SN - 1567-2379
IS - 2
ER -
ID: 10116765