Low Turnover of Soil Bacterial rRNA at Low Temperatures

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Low Turnover of Soil Bacterial rRNA at Low Temperatures. / Schostag, Morten Dencker; Albers, Christian Nyrop; Jacobsen, Carsten Suhr; Priemé, Anders.

I: Frontiers in Microbiology, Bind 11, 962, 2020.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Schostag, MD, Albers, CN, Jacobsen, CS & Priemé, A 2020, 'Low Turnover of Soil Bacterial rRNA at Low Temperatures', Frontiers in Microbiology, bind 11, 962. https://doi.org/10.3389/fmicb.2020.00962

APA

Schostag, M. D., Albers, C. N., Jacobsen, C. S., & Priemé, A. (2020). Low Turnover of Soil Bacterial rRNA at Low Temperatures. Frontiers in Microbiology, 11, [962]. https://doi.org/10.3389/fmicb.2020.00962

Vancouver

Schostag MD, Albers CN, Jacobsen CS, Priemé A. Low Turnover of Soil Bacterial rRNA at Low Temperatures. Frontiers in Microbiology. 2020;11. 962. https://doi.org/10.3389/fmicb.2020.00962

Author

Schostag, Morten Dencker ; Albers, Christian Nyrop ; Jacobsen, Carsten Suhr ; Priemé, Anders. / Low Turnover of Soil Bacterial rRNA at Low Temperatures. I: Frontiers in Microbiology. 2020 ; Bind 11.

Bibtex

@article{b73257f7e277408a883217437d8bda7c,
title = "Low Turnover of Soil Bacterial rRNA at Low Temperatures",
abstract = "Ribosomal RNA (rRNA) is used widely to investigate potentially active microorganisms in environmental samples, including soil microorganisms and other microbial communities that are subjected to pronounced seasonal variation in temperature. This raises a question about the turnover of intracellular microbial rRNA at environmentally relevant temperatures. We analyzed the turnover at four temperatures of RNA isolated from soil bacteria amended with 14C-labeled uridine. We found that the half-life of recently produced RNA increased from 4.0 days at 20°C to 15.8 days at 4°C, and 215 days at −4°C, while no degradation was detected at −18°C during a 1-year period. We discuss the implications of the strong temperature dependency of rRNA turnover for interpretation of microbiome data based on rRNA isolated from environmental samples.",
keywords = "environmental RNA, ribosomal RNA, rRNA half-life, temperature response, uridine",
author = "Schostag, {Morten Dencker} and Albers, {Christian Nyrop} and Jacobsen, {Carsten Suhr} and Anders Priem{\'e}",
note = "CENPERMOA[2020]",
year = "2020",
doi = "10.3389/fmicb.2020.00962",
language = "English",
volume = "11",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Low Turnover of Soil Bacterial rRNA at Low Temperatures

AU - Schostag, Morten Dencker

AU - Albers, Christian Nyrop

AU - Jacobsen, Carsten Suhr

AU - Priemé, Anders

N1 - CENPERMOA[2020]

PY - 2020

Y1 - 2020

N2 - Ribosomal RNA (rRNA) is used widely to investigate potentially active microorganisms in environmental samples, including soil microorganisms and other microbial communities that are subjected to pronounced seasonal variation in temperature. This raises a question about the turnover of intracellular microbial rRNA at environmentally relevant temperatures. We analyzed the turnover at four temperatures of RNA isolated from soil bacteria amended with 14C-labeled uridine. We found that the half-life of recently produced RNA increased from 4.0 days at 20°C to 15.8 days at 4°C, and 215 days at −4°C, while no degradation was detected at −18°C during a 1-year period. We discuss the implications of the strong temperature dependency of rRNA turnover for interpretation of microbiome data based on rRNA isolated from environmental samples.

AB - Ribosomal RNA (rRNA) is used widely to investigate potentially active microorganisms in environmental samples, including soil microorganisms and other microbial communities that are subjected to pronounced seasonal variation in temperature. This raises a question about the turnover of intracellular microbial rRNA at environmentally relevant temperatures. We analyzed the turnover at four temperatures of RNA isolated from soil bacteria amended with 14C-labeled uridine. We found that the half-life of recently produced RNA increased from 4.0 days at 20°C to 15.8 days at 4°C, and 215 days at −4°C, while no degradation was detected at −18°C during a 1-year period. We discuss the implications of the strong temperature dependency of rRNA turnover for interpretation of microbiome data based on rRNA isolated from environmental samples.

KW - environmental RNA

KW - ribosomal RNA

KW - rRNA half-life

KW - temperature response

KW - uridine

U2 - 10.3389/fmicb.2020.00962

DO - 10.3389/fmicb.2020.00962

M3 - Journal article

C2 - 32523564

AN - SCOPUS:85086233880

VL - 11

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 962

ER -

ID: 243852139